MR-101

Sigma-Aldrich

EmbryoMax Advanced KSOM Embryo Medium

EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.

eCl@ss:
32160801

Quality Level

form

liquid

manufacturer/tradename

EmbryoMax
Specialty Media

application(s)

cell culture | embryo: suitable
cell culture | stem cell: suitable

input

sample type: mouse embryo(s)

Related Categories

General description

EmbryoMax Advanced KSOM Medium is a new, uniquely buffered modified version of KSOM that can be used as a single medium to harvest and culture preimplantation mouse embryos. The medium will maintain pH out on the bench top and also grow embryos in vitro, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture. The Advanced KSOM medium has been shown to produce a higher frequency of blastocyst formation in multiple mouse strains after 72 hours in culture when compared to standard KSOM medium.

Traditionally, retrieving and then growing mouse embryos in vitro is usually a 2-step system. Embryos are commonly removed from the oviducts in a HEPES buffered medium (e.g. M2, FHM), which maintain their pH in air. The embryos are then transferred into a second medium which is bicarbonate buffered (e.g. M16, CZB, KSOM) and placed in a CO2 incubator in order to maintain the pH of the medium. Furthermore, the bicarbonate buffered medium needs to be pre-equilibrated in the incubator for several hours prior to receiving embryos to achieve optimal growth. The EmbryoMax Advanced KSOM Medium enables both embryo handling in atmospheric conditions and culturing in a CO2 incubator with a single medium. No medium pre-equilibration step is necessary for embryo harvest, but pre-equilibration is recommended for culture.

Application

Research Category
Stem Cell Research
EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.

Storage and Stability

Can be stored at -20°C up to the expiration date on the bottle.
Once thawed it should be used within 2 weeks.

Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.

Legal Information

EmbryoMax is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

hazcat

Skin Sens. 1

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Kenji Sugioka et al.
Developmental cell, 46(3), 257-270 (2018-07-24)
Cell division axes during development are specified in different orientations to establish multicellular assemblies, but the mechanisms that generate division axis diversity remain unclear. We show here that patterns of cell contact provide cues that diversify cell division orientation by...
Jiho Choi et al.
Cell stem cell, 20(5), 706-719 (2017-04-04)
Blastocyst-derived embryonic stem cells (ESCs) and gonad-derived embryonic germ cells (EGCs) represent two classic types of pluripotent cell lines, yet their molecular equivalence remains incompletely understood. Here, we compare genome-wide methylation patterns between isogenic ESC and EGC lines to define...
Negin P Martin et al.
Journal of visualized experiments : JoVE, (141) (2018-11-20)
Lentiviruses are efficient vectors for gene delivery to mammalian cells. Following transduction, the lentiviral genome is stably incorporated into the host chromosome and is passed on to progeny. Thus, they are ideal vectors for creation of stable cell lines, in...
Articles
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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