Yes, both MSIPS4W10 and S5EM077I10 correspond to the same plate, identified by the description: MultiScreen HTS IP PVDF 0.45um White Sterile 10pk. MSIPS4W10 is the public catalog number, whereas S5EM077I10 is a custom order request for a specific customer.
MSIPS4W10
Multiscreen® 96 well Plate, hydrophobic PVDF membrane
pore size 0.45 μm, sterile
Multiscreen® 96 well Plate, hydrophobic PVDF membrane
Synonym(s):
Hydrophobic 96-Well Plate, PVDF 96-Well Plate
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 ea | Check Cart for Availability | $452.00 |
About This Item
UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32039006
Material:
Durapore PVDF membrane, flat bottom wells, white acrylic plate
Pore size:
0.45 μm pore size
Sterility:
sterile
Binding type:
high binding surface
$452.00
Check Cart for Availability
Quality Level
material
Durapore PVDF membrane, flat bottom wells, white acrylic plate
description
Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.
sterility
sterile
usage
Automated
feature
hydrophobic, lid
packaging
pkg of 10 plates
manufacturer/tradename
MultiScreen®
technique(s)
ELISPOT: suitable, cell based assay: suitable, dot blot: suitable
H
14.4 mm
L
127.8 mm
W
85.5 mm
filtration area
0.28 cm2
plate size
96 wells
well maximum volume
300 μL
working volume
50-250 μL
matrix
Immobilon®-P
pore size
0.45 μm pore size
binding type
high binding surface
compatibility
centrifuge & manifold
shipped in
ambient
storage temp.
10-30°C
General description
The polyvinylidene fluoride (PVDF) membrane plates are recommended, over a mixed cellulose ester format, due to slightly improved binding of capture antibody and superior performance in spot detection, particularly for fluorescent applications.
Application
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| manufacturer/tradename MultiScreen® | manufacturer/tradename MultiScreen® | manufacturer/tradename MultiScreen® | manufacturer/tradename MultiScreen® |
| material Durapore PVDF membrane, white acrylic plate, flat bottom wells | material Durapore PVDF membrane, flat bottom wells, white Barex® plate (co-polymer) | material Durapore PVDF membrane, clear acrylic plate, flat bottom wells | material Durapore PVDF membrane, flat bottom wells, white Barex® plate (co-polymer) |
| binding type high binding surface | binding type low binding surface | binding type high binding surface | binding type high binding surface |
| sterility sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| feature hydrophobic, lid | feature hydrophilic, lid | feature hydrophobic, lid | feature hydrophobic, lid |
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Certificates of Analysis (COA)
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Protocols
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Articles
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
Related Content
This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.
MultiScreen® Filter Plates
Katrin Klocke et al.
Immunology, 152(1), 125-137 (2017-05-13)
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) -mediated regulation of already tolerized autoreactive T cells is critical for understanding autoimmune responses. Although defects in CTLA-4 contribute to abnormal FOXP3+ regulatory T (Treg) cell function in rheumatoid arthritis, its role in autoreactive T
Mubeen M Mosaheb et al.
Nature communications, 11(1), 524-524 (2020-01-29)
Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of
Leah E Cole et al.
Vaccines, 8(4) (2020-12-06)
While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MSIPS4W10 | 04053252464744 |
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Do MSIPS4W10 and S5EM077I10 refer to the same plate?
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Great plate for ELISPOTS
These plates give a strong ELISPOT signal with very low background and work well with either a vacuum manifold plate washing apparatus or manual washing.
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