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MSIPS4W10

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, sterile

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eCl@ss:
32039006
NACRES:
NB.22

material

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Quality Level

description

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

sterility

sterile

product line

MultiScreen®

feature

hydrophobic
lid

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

ELISPOT: suitable

filtration area

0.28 cm2

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

binding type

high binding surface

shipped in

ambient

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This Item
MSHVN4BMAIPN4550MSIPN4W50
pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

binding type

high binding surface

binding type

low binding surface

binding type

high binding surface

binding type

high binding surface

sterility

sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

manufacturer/tradename

MultiScreen®

manufacturer/tradename

MultiScreen®

manufacturer/tradename

MultiScreen®

manufacturer/tradename

MultiScreen®

well maximum volume

300 μL

well maximum volume

300 μL

well maximum volume

300 μL

well maximum volume

300 μL

General description

The polyvinylidene fluoride (PVDF) membrane plates are recommended, over a mixed cellulose ester format, due to slightly improved binding of capture antibody and superior performance in spot detection, particularly for fluorescent applications.

Application

MultiScreen® HTS IP Filter Plate, 0.45 μm, white, sterile has been used in enzyme-linked immunospot (ELISpot) assay using:
  • dsDNA-producing plasma cells
  • memory B cells and bone marrow localized long-lived plasma cells
  • draining lymph node and spleen cells

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

Protocols

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Related Content

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service