MilliporeSigma
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MT17MAG47PMX25BK

Millipore

MILLIPLEX® Mouse TH17 Magnetic Bead Panel - Premixed 25 plex Space Saver (Bulk) Packaging

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple cytokine and chemokine Th17 biomarkers in mouse serum, plasma and cell culture samples.

eCl@ss:
32161000

Quality Level

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

linearity: 111.0%
(1:04)

linearity: 113.1%
(1:08)

standard curve range: 1.5-1,500 pg/mL
(IL-4)

standard curve range: 10-10,000 pg/mL
(IL-17F)

standard curve range: 127-130,000 pg/mL
(IL-28B)

standard curve range: 15-15,000 pg/mL
(IL-1β)

standard curve range: 2.4-2,500 pg/mL
(IL-22)

standard curve range: 20-20,000 pg/mL
(IL-10, IL-12(p70), & IL-21)

standard curve range: 3.4-3,500 pg/mL
(TNFα)

standard curve range: 34-35,000 pg/mL
(GM-CSF & IL-15)

standard curve range: 342-350,000 pg/mL
(IL-23)

standard curve range: 39-40,000 pg/mL
(IL-13 & IL-17A)

standard curve range: 4.9-5,000 pg/mL
(IL-5)

standard curve range: 488-500,000 pg/mL
(TNFβ)

standard curve range: 49-50,000 pg/mL
(CD40L IL-31 & MIP-3α)

standard curve range: 586-600,000 pg/mL
(IL-17E/ IL-25)

standard curve range: 6.9-6,000 pg/mL
(IL-2)

standard curve range: 7.8-8,000 pg/mL
(IFNγ & IL-6)

standard curve range: 78-80,000 pg/mL
(IL-33)

standard curve range: 879-900,000 pg/mL
(IL-27)

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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MECY2MAG73KPXBKMCYTMAG70PMX32BKHT17MAG14PMX25BK
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

linearity: 111.0%
(1:04), linearity: 113.1%
(1:08), standard curve range: 1.5-1,500 pg/mL
(IL-4), standard curve range: 10-10,000 pg/mL
(IL-17F), standard curve range: 127-130,000 pg/mL
(IL-28B), standard curve range: 15-15,000 pg/mL
(IL-1β), standard curve range: 2.4-2,500 pg/mL
(IL-22), standard curve range: 20-20,000 pg/mL
(IL-10, IL-12(p70), & IL-21), standard curve range: 3.4-3,500 pg/mL
(TNFα), standard curve range: 34-35,000 pg/mL
(GM-CSF & IL-15), standard curve range: 342-350,000 pg/mL
(IL-23), standard curve range: 39-40,000 pg/mL
(IL-13 & IL-17A), standard curve range: 4.9-5,000 pg/mL
(IL-5), standard curve range: 488-500,000 pg/mL
(TNFβ), standard curve range: 49-50,000 pg/mL
(CD40L IL-31 & MIP-3α), standard curve range: 586-600,000 pg/mL
(IL-17E/ IL-25), standard curve range: 6.9-6,000 pg/mL
(IL-2), standard curve range: 7.8-8,000 pg/mL
(IFNγ & IL-6), standard curve range: 78-80,000 pg/mL
(IL-33), standard curve range: 879-900,000 pg/mL
(IL-27)

assay range

-

assay range

-

assay range

-

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

compatibility

configured for Premixed

compatibility

configured for Premixed

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

General description

The MILLIPLEX® Mouse Th17 Bead panel enables you to focus on the therapeutic potential of the modulation of CD4 T-helper cell cytokines. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
There was no or minimum cross-reactivity between the antibodies and any other analytes in the panel (see details in assay protocol).
UPDATE >>
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: CD40 Ligand, GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27, IL-28B, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ
  • Recommended Sample type: serum, plasma or tissue/cell lysate and culture supernatant
  • Recommended Sample dilution: neat
  • Assay Run Time: overnight or one day
  • Research Category: Inflammation & Immunology

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Yu-Wen Liu et al.
JCI insight, 7(11) (2022-05-04)
Positive regulatory domain 1 (PRDM1) encodes B lymphocyte-induced maturation protein 1 (BLIMP1), also known as a master regulator of T cell homeostasis. We observed a negative relationship between Blimp-1 and IL-21 based on our previous data that Blimp-1 overexpression in

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