PC05L

Sigma-Aldrich

Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

lyophilized, Calbiochem®

storage conditions

+2C to +8C

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

does not contain

preservative

species reactivity

mouse, human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

ambient

storage temp.

2-8°C

General description

This Anti-c-Fos (Ab-2) (4-17) Rabbit pAb is validated for use in Frozen Sections, WB, IF, IP, Paraffin Sections, Gel Shift, for the detection of c-Fos (Ab-2) (4-17).
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~50-62 kDa c-Fos protein.
The proto-oncogene fos has been implicated in cell growth, differentiation and development. fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
Recognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.

Immunogen

a synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
Human

Packaging

100 μg in Glass bottle

Application

Frozen Sections (5-10 µg/ml)

Immunoblotting (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg per reaction)

Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)

Gel Shift (see comments and application references)

Free-floating Sections (not recommended)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from PBS, a volatile buffer, 100 µg BSA.

Reconstitution

Resuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.

Analysis Note

Positive Control
Hep G2 or NIH 3T3 cells

Other Notes

DeTogni, P., et al. 1988. Cell Biol.8, 2251.
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell51, 513.
Muller, R. 1986. Biochim. Biophys. Acta823, 207.
Verma, I. 1986. Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433
For gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Cellular and viral fos genes: structure, regulation of expression and biological properties of their encoded products.
R Müller
Biochimica et biophysica acta, 823(3), 207-225 (1986-01-01)
P De Togni et al.
Molecular and cellular biology, 8(5), 2251-2256 (1988-05-01)
We have generated monoclonal antibodies by using a synthetic peptide corresponding to amino acid positions 4 to 17 of the human fos protein. The antibodies detected both v- and c-fos proteins by immunoprecipitation, immunoblotting, and indirect immunofluorescence. The monoclonal antibodies...
Proto-oncogene fos: complex but versatile regulation.
I M Verma et al.
Cell, 51(4), 513-514 (1987-11-20)
M E Greenberg et al.
Nature, 311(5985), 433-438 (1984-10-04)
Transcription of the c-fos proto-oncogene is greatly increased within minutes of administering purified growth factors to quiescent 3T3 cells. This stimulation is the most rapid transcriptional response to peptide growth factors yet described, and implies a role for c-fos in...
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