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Anti-Rad51 (Ab-1) Rabbit pAb

liquid, Calbiochem®

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Select a Size

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, rat, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... RAD51(5888)

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This Item
SAB5700826ABE257SAB4504264
Anti-RAD51 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABE257

Anti-RAD51 Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

liquid

form

buffered aqueous solution

form

-

form

buffered aqueous solution

species reactivity

mouse, rat, human

species reactivity

rat, mouse, human

species reactivity

rat, human, mouse

species reactivity

rat, human, mouse

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

General description

Affinity purified rabbit polyclonal antibody. Recognizes the ~43 kDa Rad51 protein.
Recognizes the ~43 kDa Rad51 protein in ovarian tissue.
The bacterial RecA and the yeast RAD51 proteins are involved in homologous recombination and DNA repair. The human RAD51 protein is the homologue of these two proteins. While the modular structure of these proteins is conserved and the homology is very strong with the bacterial protein there are functional differences seen with RAD51 including an ATP hydrolysis rate 1/10 that seen with RecA. Although RAD51 is a nuclear protein and is largely absent from the cytoplasm and nucleoli, the protein lacks a nuclear localization signal and requires other proteins in a complex to be translocated into the nucleus. Treatment of cells with DNA synthesis inducing agents will cause an increase in the levels of RAD51 protein which then undergo a shift in distribution becoming focal in nature. This focal distribution may be blocked by inhibitors of transcription. RAD51 can be shown to interact directly with the chromosomes and during the process of meiosis, its distribution of RAD51 undergoes dynamic change. RAD51 has been shown to interact with a wide variety of different proteins that include the wild-type form of p53, BRCA2, and RAD52, which specifically modulates its activity.
This Anti-Rad51 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Rad51 (Ab-1).

Immunogen

Human
recombinant, human Rad51

Application




Immunoblotting (1:2500)
Immunoprecipitation (40 l/500g total protein from lysate)
Immunocytochemistry (1:500)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
A431 cells, HeLa cells

Other Notes

Brendel, V., et al. 1997. J. Mol. Evol.44, 528.
Boulikas, T., et al. 1997. Anticancer Res.17, 843.
Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA94, 463.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
Sharan, S.K., et al. 1997. Nature386, 304.
Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10222.
Shen, Z., et al. 1996. J. Biol. Chem.271, 148.
Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
Tashiro, S., et al. 1996. Oncogene12, 2165.
Ashley, T., et al. 1995. Chromosoma104, 19.
Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA92, 2298.
Due to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Or David Shahar et al.
Nucleic acids research, 42(9), 5689-5701 (2014-04-01)
DNA double-strand breaks (DSBs) are the most severe type of DNA damage. DSBs are repaired by non-homologous end-joining or homology directed repair (HDR). Identifying novel small molecules that affect HDR is of great importance both for research use and therapy.
Céline Jacquemont et al.
Molecular cancer, 11, 26-26 (2012-04-28)
Platinum compounds such as cisplatin and carboplatin are DNA crosslinking agents widely used for cancer chemotherapy. However, the effectiveness of platinum compounds is often tempered by the acquisition of cellular drug resistance. Until now, no pharmacological approach has successfully overcome
Rachel O'Dea et al.
Journal of cell science, 133(3) (2020-01-23)
In order to prevent the deleterious effects of genotoxic agents, cells have developed complex surveillance mechanisms and DNA repair pathways that allow them to maintain genome integrity. The ubiquitin-specific protease 9X (USP9X) contributes to genome stability during DNA replication and
Yue Sun et al.
Cell death & disease, 11(4), 292-292 (2020-04-29)
Genome instability is the fundamental hallmark of malignant tumors. Tumor suppressors often play a role in maintaining genome stability. Our previous genetic screen identified inositol polyphosphate 4-phosphatase type B (INPP4B), primarily hydrolyzing phosphatidylinositol 3, 4-disphosphate, is a potential tumor suppressor
Nuria Coll-Bonfill et al.
Proteomics, 20(5-6), e1800406-e1800406 (2019-12-14)
Hutchinson Gilford progeria syndrome (HGPS) is a devastating accelerated aging disease caused by LMNA gene mutation. The truncated lamin A protein produced "progerin" has a dominant toxic effect in cells, causing disruption of nuclear architecture and chromatin structure, genomic instability

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