MilliporeSigma
All Photos(1)

Documents

PC635

Sigma-Aldrich

PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb

liquid, Calbiochem®

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-Nibrin (pSer³⁴³) Rabbit pAb

biological source

rabbit

Quality Level

antibody form

diluted serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer343)

Gene Information

human ... NBN(4683)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
AP1064PS1019DR1025
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

Gene Information

human ... NBN(4683)

Gene Information

-

Gene Information

human ... MAPK8(5599)

Gene Information

human ... CHEK1(1111)

species reactivity

human

species reactivity

human

species reactivity

mouse, rat, human

species reactivity

monkey, rat, mouse, human

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser343.
Recognizes the ~95 kDa nibrin protein phosphorylated at Ser343 in δ-irradiated lymphoblastoid cells.
This PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).

Immunogen

Human
a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid

Application

ELISA (1:16,000)

Immunoblotting (1:1000)

Immunoprecipitation (1 µl/500 µg cell extract)

Warning

Toxicity: Standard Handling (A)

Physical form

Serum diluted in PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
γ-irradiated lymphoblastoid cells

Other Notes

Gatei, M., et al. 2000. Nat. Genet.25, 115.
Lim, D.S., et al. 2000. Nature404, 613.
Wu, G., et al. 2000. J. Biol. Chem.275, 30618.
Zhong, Q., et al. 1999. Science285, 747.
Carney, J.P., et al. 1998. Cell93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem.273, 21447.
Varon, R., et al. 1998. Cell93, 467.
This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service