PC709

Anti-MYH (33-51) Rabbit pAb

liquid, Calbiochem^®

• Synonym(s): Anti-MutY Homolog

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MUTYH(4595)

Description

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~52-57 kDa isoforms of MYH.

Recognizes the MYH protein and several of its approximately 10 isoforms in PC12, Jurkat, and HeLa cells.

This Anti-MYH (33-51) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections for the detection of MYH (33-51).

Immunogen

Human

a synthetic peptide (QEGRQKHAKNNSQAKPSAC) corresponding to amino acids 33-51 of human MYH

Application

Immunoblotting (1:1500)

Immunofluorescence (1:500)

Paraffin Sections (see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
PC12, Jurkat, or HeLa cells

Other Notes

Detects MYH and several of its approximately ten isoforms. In Jurkat cells the ~52 kDa and ~53 kDa variants were found in the nuclear fraction while the ~57 kDa protein is found in the mitochondria. Antibody should be titrated for optimal results in individual systems.

Wang, G., et al. 2000. Nucleic Acids Res.28, 2135.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable