MilliporeSigma
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PCYTMAG-23K

Millipore

MILLIPLEX® Porcine Cytokine and Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology in porcine serum, plasma and cell culture samples.

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Synonym(s):
luminex porcine cytokine chemokine growth factor multiplex assay, luminex porcine pro inflammatory anti inflammatory cytokine panel, millipore porcine inflammation multiplex kit, porcine interleukin immunoassay panel
eCl@ss:
32161000

Quality Level

species reactivity

pig

manufacturer/tradename

Milliplex®

assay range

accuracy: 92-103%
standard curve range: 0.005-20 ng/mL
(IL-1α)

standard curve range: 0.012-50 ng/mL
(IL-8)

standard curve range: 0.024-100 ng/mL
(GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18 & TNFα)

standard curve range: 0.061-250 ng/mL
(IL-4)

standard curve range: 0.122-500 ng/mL
(IFNγ & IL-1β)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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This Item
PCYTMG-23K-13PXCCYTMG-90K-PX13CCYTOMAG-90K
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 92-103%, standard curve range: 0.005-20 ng/mL
(IL-1α), standard curve range: 0.012-50 ng/mL
(IL-8), standard curve range: 0.024-100 ng/mL
(GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18 & TNFα), standard curve range: 0.061-250 ng/mL
(IL-4), standard curve range: 0.122-500 ng/mL
(IFNγ & IL-1β)

assay range

accuracy: 92-103%, standard curve range: 0.005-20 ng/mL
(IL-1α), standard curve range: 0.012-50 ng/mL
(IL-8), standard curve range: 0.024-100 ng/mL
(GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18 & TNFα), standard curve range: 0.061-250 ng/mL
(IL-4), standard curve range: 0.122-500 ng/mL
(IFNγ & IL-1β)

assay range

accuracy: 91-109%, sensitivity: 13.6 pg/mL
(IFN-γ), sensitivity: 21.0 pg/mL
(MCP-1), sensitivity: 21.7 pg/mL
(IL-8), sensitivity: 3.2 pg/mL
(IP-10), sensitivity: 3.5 pg/mL
(IL-2), sensitivity: 3.7 pg/mL
(IL-6), sensitivity: 5.3 pg/mL
(KC-like), sensitivity: 5.8 pg/mL
(IL-18), sensitivity: 6.1 pg/mL
(TNFα), sensitivity: 7.5 pg/mL
(IL-7), sensitivity: 8.5 pg/mL
(IL-10), sensitivity: 9.0 pg/mL
(IL-15), sensitivity: 9.2 pg/mL
(GM-CSF), standard curve range: 12.2-50,000 pg/mL
(All analytes)

assay range

accuracy: 91-109%, sensitivity: 13.6 pg/mL
(IFN-γ), sensitivity: 21.0 pg/mL
(MCP-1), sensitivity: 21.7 pg/mL
(IL-8), sensitivity: 3.2 pg/mL
(IP-10), sensitivity: 3.5 pg/mL
(IL-2), sensitivity: 3.7 pg/mL
(IL-6), sensitivity: 5.3 pg/mL
(KC-like), sensitivity: 5.8 pg/mL
(IL-18), sensitivity: 6.1 pg/mL
(TNFα), sensitivity: 7.5 pg/mL
(IL-7), sensitivity: 8.5 pg/mL
(IL-10), sensitivity: 9.0 pg/mL
(IL-15), sensitivity: 9.2 pg/mL
(GM-CSF), standard curve range: 12.2-50,000 pg/mL
(All analytes)

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

With a similarity to humans in heart size, blood flow rate, skin, liver enzymes and bone healing, porcine models have replaced many canine and non-human primate models in many research areas such as cardiovascular, toxicology, pharmacology, aging and bone. “Minipigs,” bred to retain the similar heart size and function as humans, but age without growing too large, have been used for long term preclinical or multi-generational studies which may be performed in a comparatively short period of time.

MILLIPLEX® Porcine Cytokine/Chemokine Panel can be used for the simultaneous quantification of any or all of the following analytes in porcine tissue/cell lysate and culture supernatant samples and serum or plasma samples: GM-CSF, IFNγ, IL-1α, IL-1ra, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: GM-CSF, IFNγ, IL-1α, IL-1β, IL-1Ra, IL-2, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-12, IL-18, TNF-α
  • Recommended Sample Type: serum, plasma or cell/tissue culture supernatants or lysate
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • NOTE: Extra centrifugation and attention may be needed for samples with extremely high lipid content.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C.
  • Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3


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Christina Veit et al.
Frontiers in veterinary science, 7, 617634-617634 (2021-02-16)
Poor health is a risk factor for damaging behaviors, but the mechanisms behind this link are unknown. Injection of pigs with lipopolysaccharide (LPS) can be used to model aspects of poor health. Recent studies have shown that LPS-injected pigs perform
Victoria J Vieira-Potter et al.
Obesity (Silver Spring, Md.), 23(12), 2421-2429 (2015-11-03)
The Ossabaw pig is emerging as an attractive model of human cardiometabolic disease because of its size and susceptibility to atherosclerosis, among other characteristics. The relationship between adipose tissue inflammation and metabolic dysfunction in this model was investigated here. Young
Marina López et al.
Animals : an open access journal from MDPI, 11(10) (2021-10-24)
This work studied the effects of the inclusion of Clostridium butyricum on feed, alone or with carob meal or citrus pulp, on the digestive and metabolic status of weaned piglets. A total of 30 male piglets (weaned at 21 days)
Ruipeng Zhao et al.
International immunopharmacology, 99, 107905-107905 (2021-07-10)
To determine whether idealized anterior cruciate ligament reconstruction (IACL-R) restores normal gait features, and whether inflammatory factors are involved in the pathogenesisof post-traumatic osteoarthritis (PTOA). Fourteen mature female minipigs were allocated to a sham group (n = 7) or an IACL-R group
David S Gardner et al.
American journal of physiology. Renal physiology, 310(4), F259-F271 (2015-11-27)
Acute kidney injury (AKI) is a common and serious condition with no specific treatment. An episode of AKI may affect organs distant from the kidney, further increasing the morbidity associated with AKI. The mechanism of organ cross talk after AKI

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