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PCYTMG-23K-13PX

Millipore

MILLIPLEX® Porcine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in porcine serum, plasma and cell culture samples.

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Synonym(s):
luminex porcine cytokine chemokine growth factor multiplex assay, luminex porcine pro inflammatory anti inflammatory cytokine panel, millipore porcine inflammation multiplex kit, porcine interleukin immunoassay panel
eCl@ss:
32161000

Quality Level

species reactivity

pig

manufacturer/tradename

Milliplex®

assay range

accuracy: 92-103%
standard curve range: 0.005-20 ng/mL
(IL-1α)

standard curve range: 0.012-50 ng/mL
(IL-8)

standard curve range: 0.024-100 ng/mL
(GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18 & TNFα)

standard curve range: 0.061-250 ng/mL
(IL-4)

standard curve range: 0.122-500 ng/mL
(IFNγ & IL-1β)

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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This Item
PCYTMAG-23KPCYTMAG23PMX13BKCCYTOMAG-90K
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 92-103%, standard curve range: 0.005-20 ng/mL
(IL-1α), standard curve range: 0.012-50 ng/mL
(IL-8), standard curve range: 0.024-100 ng/mL
(GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18 & TNFα), standard curve range: 0.061-250 ng/mL
(IL-4), standard curve range: 0.122-500 ng/mL
(IFNγ & IL-1β)

assay range

accuracy: 92-103%, standard curve range: 0.005-20 ng/mL
(IL-1α), standard curve range: 0.012-50 ng/mL
(IL-8), standard curve range: 0.024-100 ng/mL
(GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18 & TNFα), standard curve range: 0.061-250 ng/mL
(IL-4), standard curve range: 0.122-500 ng/mL
(IFNγ & IL-1β)

assay range

-

assay range

accuracy: 91-109%, sensitivity: 13.6 pg/mL
(IFN-γ), sensitivity: 21.0 pg/mL
(MCP-1), sensitivity: 21.7 pg/mL
(IL-8), sensitivity: 3.2 pg/mL
(IP-10), sensitivity: 3.5 pg/mL
(IL-2), sensitivity: 3.7 pg/mL
(IL-6), sensitivity: 5.3 pg/mL
(KC-like), sensitivity: 5.8 pg/mL
(IL-18), sensitivity: 6.1 pg/mL
(TNFα), sensitivity: 7.5 pg/mL
(IL-7), sensitivity: 8.5 pg/mL
(IL-10), sensitivity: 9.0 pg/mL
(IL-15), sensitivity: 9.2 pg/mL
(GM-CSF), standard curve range: 12.2-50,000 pg/mL
(All analytes)

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

compatibility

-

compatibility

configured for Premixed

compatibility

-

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

General description

With a similarity to humans in heart size, blood flow rate, skin, liver enzymes and bone healing, porcine models have replaced many canine and non-human primate models in many research areas such as cardiovascular, toxicology, pharmacology, aging and bone. “Minipigs,” bred to retain the similar heart size and function as humans, but age without growing too large, have been used for long term preclinical or multi-generational studies which may be performed in a comparatively short period of time.

The MILLIPLEX® Porcine Cytokine/Chemokine Panel can be used for the simultaneous quantification of all the following analytes in porcine tissue/cell lysate and culture supernatant samples and serum or plasma samples: GM-CSF, IFNγ, IL-1α, IL-1ra, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: GM-CSF, IFNγ, IL-1α, IL-1β, IL-1Ra, IL-2, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-12, IL-18, TNF-α
  • Recommended Sample Type: serum, plasma or cell/tissue culture supernatants or lysate
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • NOTE: Extra centrifugation and attention may be needed for samples with extremely high lipid content.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C.
  • Research Category: Inflammation & Immunology

Packaging

Premixed beads for your convenience.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3


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Song-Song Xu et al.
Frontiers in nutrition, 9, 807118-807118 (2022-03-15)
The diet-induced gut microbiota dysbiosis has been suggested as a major risk factor for atherothrombosis, however, the detailed mechanism linking these conditions is yet to be fully understood. We established a long-term excessive-energy diet-induced metabolic syndrome (MetS) inbred Wuzhishan minipig
Lorena Padilla et al.
Biology, 9(5) (2020-05-24)
The concentrations of cytokines in seminal plasma (SP) fluctuate over time in healthy males, weakening their practical usefulness as diagnostic tools. This study evaluated the relevance of intra-male variability in SP cytokines and to what extent the period of the
Rahul K Nelli et al.
mSphere, 6(6), e0082021-e0082021 (2021-12-23)
The upper respiratory tract is the primary site of infection by porcine hemagglutinating encephalomyelitis virus (PHEV). In this study, primary porcine respiratory epithelial cells (PRECs) were cultured in an air-liquid interface (ALI) to differentiate into a pseudostratified columnar epithelium, proliferative
Isabel Barranco et al.
Frontiers in veterinary science, 6, 436-436 (2019-12-24)
Background: Boar seminal plasma is rich in cytokines, which could influence the capability of spermatozoa to tolerate preservation. Objectives: To evaluate the involvement of boar seminal plasma cytokines in the changes experienced by boar spermatozoa during their storage, either in
Cristina A Martinez et al.
Biology, 10(2) (2021-03-07)
Seminal plasma (SP) in the female genital tract induces changes that affect multiple reproductive processes. One of the active components in SP is the transforming growth factor β1 (TGF-β1), which has major roles in embryo development and pregnancy. Embryo transfer

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