MilliporeSigma
All Photos(1)

Documents

PF019

Sigma-Aldrich

TIMP-1, Human, Recombinant, CHO Cells

Recombinant, human TIMP-1 expressed in CHO cells.

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
Tissue Inhibitor of Metalloproteinase-1

Quality Level

Assay

≥95% (prior to lyophilization, SDS-PAGE)

form

lyophilized

does not contain

preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
612080612084SRP3173
Sigma-Aldrich

Sigma-Aldrich

SRP3173

TIMP-1 human

form

lyophilized

form

liquid

form

liquid

form

lyophilized

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

storage condition

OK to freeze

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

-

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

General description

Recombinant, human TIMP-1 expressed in CHO cells. Migrates as a 28 kDa protein under reducing conditions on SDS-PAGE.
Recombinant, human TIMP-1 expressed in CHO cells. Migrates as a ~28 kDa protein by SDS-PAGE. The activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a ~28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.

Application

Immunoblotting Standard (20 µl/lane)

MMP Inhibition (assay dependent, see comments)

Biochem/physiol Actions

Cell permeable: no
IC₅₀ against native human MMP-1 is ~1-5 nM.
Primary Target
Human MMP 1
Product does not compete with ATP.
Reversible: no
Target IC50: 1-5 nM against human MMP 1

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from ammonium bicarbonate, sodium chloride, sodium phosphate buffer, 100 µg BSA, pH 7.3.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Reconstitute with PBS to the desired concentration and mix gently until completely dissolved.

Other Notes

Freudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm.171, 250.
Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.
Kodama, S. et al. 1987. Collagen Rel. Res.7, 341.
Docherty, A.J.P. et al. 1985. Nature318, 66.
Nagai, Y., et al. 1984. Jpn. J. Inflam.4, 123.
TIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC50 of 1-5 nM (see application references).

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service