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PF021

Sigma-Aldrich

TIMP-2, Human, Recombinant, CHC Cells

Recombinant, human TIMP-2 expressed in CHC cells.

Synonym(s):

Tissue Inhibitor of Metalloproteinase 2

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About This Item

UNSPSC Code:
12352202

Quality Level

assay

>98% (SDS-PAGE)

form

lyophilized

does not contain

preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−20°C

General description

M. W.: 24000
Recombinant, human TIMP-2 expressed in CHC cells. Migrates as a 24 kDa protein under reducing conditions on SDS-PAGE. The activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-2 is a ~21 kDa (nonreduced) or a ~24 kDa (reduced) protein on SDS-PAGE that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-2 preferentially binds and inhibits MMP-2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. Useful for SDS-PAGE/immunoblot standard and inhibition studies.
Recombinant, human TIMP-2 expressed in CHC cells. Migrates as a 24 kDa protein under reducing conditions on SDS-PAGE. Use 20 µl per gel lane.

Biochem/physiol Actions

Cell permeable: no
IC₅₀ against native human MMP-1 is in the range of 2-10 nM
Primary Target
Human MMP-1
Product does not compete with ATP.
Reversible: no
Target IC50: 2-10 nM against human MMP-1

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from PBS, 100 µg BSA.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Reconstitute by adding 200 µl PBS to the tube and mixing gently until in solution.

Other Notes

Clermont, A., et al. 2004. Biochem. J.382, 315.
Boone, T.C., et al. 1990. Proc. Natl. Acad. Sci. USA87, 2800.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology. Vol. 1(2), 99.
Kodama, S., et al. 1987. Collagen Rel. Res.7, 341.
Docherty, A.J.P., et al. 1985. Nature318, 66.

Legal Information

Sold under license of U.S. Patents 5,595,885 and 5,698,671. Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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