Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.

tissue culture plate, tissue culture insert, permeable culture insert, cell culture inserts

Quality Level


PTFE membrane (hydrophilic)
flat wells




lid: no


pack of 50




50 °C max. temp.


cell analysis: suitable (cell attachment)
cell analysis: suitable (cell differentiation)
cell culture | mammalian: suitable


13 mm


3.15 cm

filter diam.

30 mm

filtration area

4.2 cm2

plate size

1 wells

working volume

1.5 mL



pore size

0.4 μm pore size


sample type cardiac stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)

surface area

4.2 cm2

binding type

low binding surface

detection method


shipped in


General description

Device Configuration: 6-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies

Membrane Type:
Biopore Membrane (hydrophilic PTFE)
- For low protein-binding, live cell viewing, and immunofluorescent applications

Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry


50 pk in Individually blister packaged
Individually blister packaged


Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.
Research Category
Cell Culture

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
CB1 receptors and post-ischemic brain damage: Studies on the toxic and neuroprotective effects of cannabinoids in rat organotypic hippocampal slices.
Landucci, Elisa, et al.
Neuropharmacology, 60, 674-682 (2011)
Synapse elimination in olivo-cerebellar explants occurs during a critical period and leaves an indelible trace in Purkinje cells.
Letellier, Mathieu, et al.
Proceedings of the National Academy of Sciences of the USA, 106, 14102-14107 (2009)
Daniela Buonvicino et al.
British journal of pharmacology, 177(14), 3342-3356 (2020-03-22)
Drugs able to counteract progressive multiple sclerosis (MS) represent a largely unmet therapeutic need. Even though the pathogenesis of disease evolution is still obscure, accumulating evidence indicates that mitochondrial dysfunction plays a causative role in neurodegeneration and axonopathy in progressive...
Preparation of organotypic hippocampal slice cultures: interface method.
De Simoni, Anna and Yu, Lily M Y
Nature Protocols, 1, 1439-1445 (2006)
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane...
This protocol covers 3 modes for the microscopic examination of cell samples.
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This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
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This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
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