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PICM03050

Millipore

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.

Synonym(s):
tissue culture plate, tissue culture insert, permeable culture insert, cell culture inserts
eCl@ss:
32011202
NACRES:
NB.22

Quality Level

material

PTFE membrane (hydrophilic)
flat wells
polystyrene

sterility

sterile

feature

lid: no

packaging

pack of 50

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

application(s)

cell analysis: suitable (cell attachment)
cell analysis: suitable (cell differentiation)
cell culture | mammalian: suitable

H

13 mm

diam.

3.15 cm

filter diam.

30 mm

filtration area

4.2 cm2

plate size

1 wells

working volume

1.5 mL

matrix

Biopore

pore size

0.4 μm pore size

input

sample type cardiac stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)

surface area

4.2 cm2

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

General description

Device Configuration: 6-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies

Membrane Type:
Biopore Membrane (hydrophilic PTFE)
- For low protein-binding, live cell viewing, and immunofluorescent applications

Applications
Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry

Packaging

50 pk in Individually blister packaged
Individually blister packaged

Application

Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.
Research Category
Cell Culture

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
CB1 receptors and post-ischemic brain damage: Studies on the toxic and neuroprotective effects of cannabinoids in rat organotypic hippocampal slices.
Landucci, Elisa, et al.
Neuropharmacology, 60, 674-682 (2011)
Synapse elimination in olivo-cerebellar explants occurs during a critical period and leaves an indelible trace in Purkinje cells.
Letellier, Mathieu, et al.
Proceedings of the National Academy of Sciences of the USA, 106, 14102-14107 (2009)
Daniela Buonvicino et al.
British journal of pharmacology, 177(14), 3342-3356 (2020-03-22)
Drugs able to counteract progressive multiple sclerosis (MS) represent a largely unmet therapeutic need. Even though the pathogenesis of disease evolution is still obscure, accumulating evidence indicates that mitochondrial dysfunction plays a causative role in neurodegeneration and axonopathy in progressive...
Preparation of organotypic hippocampal slice cultures: interface method.
De Simoni, Anna and Yu, Lily M Y
Nature Protocols, 1, 1439-1445 (2006)
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane...
Protocols
This protocol covers 3 modes for the microscopic examination of cell samples.
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This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
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This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
Read More
Related Content
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
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