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PICM0RG50

Millipore

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.

Synonym(s):
PICMORG50, tissue culture plate, tissue culture insert, permeable culture insert, cell culture inserts
eCl@ss:
32011202
NACRES:
NB.22

material

PTFE membrane (hydrophilic)
flat wells
polystyrene

Quality Level

sterility

sterile

feature

lid: no

packaging

pack of 48

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

application(s)

cell analysis: suitable (cell attachment)
cell analysis: suitable (cell differentiation)
cell culture | mammalian: suitable

H

5 mm

diam.

3.15 cm

filter diam.

30 mm

filtration area

4.2 cm2

plate size

1 wells

matrix

Biopore

pore size

0.4 μm pore size

input

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
sample type cardiac stem cell(s)

surface area

4.2 cm2

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

General description

Device Configuration: 6-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Organotypic Insert:
- For high cell viability and superior study of three dimensional explant structure
- Lower height allows them to fit inside a standard petri dish
- The Biopore (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
- The membrane is optically clear and optimized for long-term organotypic explant maintenance

Membrane Type:
Biopore Membrane (hydrophilic PTFE)
- For low protein-binding, live cell viewing, and immunofluorescent applications

Applications
Cell Attachment, 3D Cell Culture, Cell Growth, Cell Differentiation , Immunocytochemistry

Stem Cell Types:
Mouse Embryonic Stem Cells
Human Embryonic Stem Cells
Mesenchymal Stem Cells
Neural Stem Cells
Hematopoietic Stem Cells
Epithelial Cells
Pancreatic Stem Cells
Cardiac Stem Cells
Induced Pluripotent Stem Cells

Packaging

50 pk in Individually blister packaged
Individually blister packaged

Application

Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.
Research Category
Cell Culture

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificate of Analysis
Certificate of Origin
Ryota Tamura et al.
Human gene therapy, 31(5-6), 352-366 (2020-02-23)
Glioblastoma is the most aggressive brain tumor characterized by diffuse infiltration into the normal brain parenchyma. Neural stem cells are known to possess the tumor-tropic migratory capacity and thus can be used as cellular vehicles for targeted delivery of therapeutic...
Keisuke Ikegami et al.
Investigative ophthalmology & visual science, 61(3), 26-26 (2020-03-18)
Elevated IOP can cause the development of glaucoma. The circadian rhythm of IOP depends on the dynamics of the aqueous humor and is synchronized with the circadian rhythm pacemaker, that is, the suprachiasmatic nucleus. The suprachiasmatic nucleus resets peripheral clocks...
Abigail K Myers et al.
Development (Cambridge, England), 147(2) (2020-01-10)
The precise migration of cortical interneurons is essential for the formation and function of cortical circuits, and disruptions to this key developmental process are implicated in the etiology of complex neurodevelopmental disorders, including schizophrenia, autism and epilepsy. We have recently...
Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight.
Spooner, B S, et al.
The Journal of Experimental Zoology, 269, 212-222 (1994)
James Stallock et al.
Development (Cambridge, England), 130(26), 6589-6597 (2003-12-09)
In the mouse embryo, significant numbers of primordial germ cells (PGCs) fail to migrate correctly to the genital ridges early in organogenesis. These usually die in ectopic locations. In humans, 50% of pediatric germ line tumors arise outside the gonads...
Protocols
This is a Toluidine Blue Staining protocol listing materials and methods.
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This protocol covers 3 modes for the microscopic examination of cell samples.
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This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
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This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
Read More
Related Content
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
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