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MilliporeSigma

PMEF-CFX

Sigma-Aldrich

EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Irradiated, Passage 3

Synonym(s):

CF-1 MEFs, CF-1 PMEFs, Irradiated MEFs, MEF Feeder Cells, MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells, Irradiated MEFS, Mouse Feeder Cells

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5 VIALS
$512.00

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5 VIALS
$512.00

About This Item

UNSPSC Code:
41106509
eCl@ss:
32011203
NACRES:
NA.81

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$512.00


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Technical Service
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biological source

mouse

Quality Level

manufacturer/tradename

Specialty Media
EmbryoMax®

technique(s)

cell culture | stem cell: suitable

input

sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)

shipped in

liquid nitrogen

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This Item
PMEF-DR4X-MPMEF-CFLPMEF-DR4-M
technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

shipped in

liquid nitrogen

shipped in

-

shipped in

liquid nitrogen

shipped in

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

input

sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)

input

-

input

sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)

input

-

General description

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
These PMEF cells were isolated from CF-1 mouse embryos at day 13 and mitotically inactivated by treatment with irradiation. The cells can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:

• 6 x 60mm dishes
• 2 x T75 flasks
• 2 x 6 well plates

Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells.

Cell Line Description

Primary Mouse Embryo Fibroblasts

Application

Research Category
Stem Cell Research

Packaging

5-6x106 ea

Storage and Stability

Vials should be stored in the vapor phase of liquid nitrogen.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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