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PhosphoDetect Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)

liquid, clone C25A9, Calbiochem®

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biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

C25A9, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer423/pSer425)

Gene Information

human ... SMAD3(4088)

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AP1063AP1064361527
antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

human

species reactivity

mouse, human

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

clone

C25A9, monoclonal

clone

polyclonal

clone

polyclonal

clone

2D3, monoclonal

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

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General description

Purified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425.
Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425 in HT-1080 cells treated with TGF-β1.
This PhosphoDetect Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer423/425).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH.

Application


Immunoblotting (1:1000)
Immunoprecipitation (1:50)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.

Other Notes

Attisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.
Does not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Amy M Gancarz et al.
Nature neuroscience, 18(7), 959-961 (2015-06-02)
Activin receptor signaling, including the transcription factor Smad3, was upregulated in the rat nucleus accumbens (NAc) shell following withdrawal from cocaine. Direct genetic and pharmacological manipulations of this pathway bidirectionally altered cocaine seeking while governing morphological plasticity in NAc neurons.
Beatriz Ballester et al.
International journal of molecular sciences, 22(12) (2021-07-03)
Several transmembrane mucins have demonstrated that they contribute intracellularly to induce fibrotic processes. The extracellular domain of MUC16 is considered as a biomarker for disease progression and death in IPF patients. However, there is no evidence regarding the signalling capabilities
Javier Milara et al.
Respiratory research, 23(1), 313-313 (2022-11-16)
Pulmonary hypertension (PH) associated to idiopathic pulmonary fibrosis (IPF) portends a poor prognosis. IL-11 has been implicated in fibrotic diseases, but their role on pulmonary vessels is unknown. Here we analyzed the contribution of IL-11 to PH in patients with
Javier Milara et al.
Mucosal immunology, 14(2), 377-388 (2020-09-06)
Several mucins are implicated in idiopathic pulmonary fibrosis (IPF); however, there is no evidence regarding the role of MUC4 in the development of IPF. Here we demonstrated that MUC4 was overexpressed in IPF patients (n = 22) compared with healthy subjects (n = 21)
Beatriz Ballester et al.
Oncotarget, 11(15), 1306-1320 (2020-04-29)
Pirfenidone is a pleiotropic molecule approved to treat idiopathic pulmonary fibrosis (IPF). Pirfenidone has demonstrated to downregulate transforming growth factor-β1 (TGF-β1) cellular effects. However, its anti-fibrotic mechanism remains unclear. Here, we aim to analyze the effects of pirfenidone on the

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