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PSHT004S5

Millipore

Millicell® 96 well Plate

pore size 4.0 μm, polycarbonate membrane, sterile

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eCl@ss:
32039006
NACRES:
NB.13

material

flat wells
polycarbonate membrane
polystyrene

Quality Level

description

Polycarbonate, 0.4 μm pore size, sterile, 96-well cell culture plate, 96-well receiver tray and lid, pack of 5

sterility

sterile

feature

lid

manufacturer/tradename

Millicell®

parameter

75-100 μL sample volume

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

H

26.2 mm

L

128 mm

W

84.8 mm

filtration area

0.11 cm2

plate size

96 wells

working volume

75 μL

matrix

Isopore

pore size

0.4 μm pore size
4.0 μm pore size

input

sample type: human embryonic stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type cardiac stem cell(s)

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

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PSMT010RPSST010RPSET010
vibrant-m

PSHT004S5

Millicell® 96 well Plate

vibrant-m

PSET010

Millicell® 24 well Plate

pore size

0.4 μm pore size, 4.0 μm pore size

pore size

5.0 μm pore size

pore size

3.0 μm pore size

pore size

8.0 μm pore size

binding type

Tissue Culture (TC)-treated surface

binding type

Tissue Culture (TC)-treated surface

binding type

Tissue Culture (TC)-treated surface

binding type

Tissue Culture (TC)-treated surface

sterility

sterile

sterility

sterile

sterility

sterile

sterility

sterile

material

flat wells, polystyrene , polycarbonate membrane

material

flat wells, polycarbonate membrane, polystyrene

material

flat wells, polycarbonate membrane, polystyrene

material

flat wells, polycarbonate membrane, polystyrene

feature

lid

feature

lid

feature

lid

feature

lid

General description

Filter Code: PCF
Millicell cell culture insert plates are a patented device designed to support cell growth and differentiation of endothelial and epithelial cell lines, including CaCo-2.

The Millicell device is available with a 0.4 µm PCF and a variety of PET membrane pore sizes. The plate is designed for maximum user convenience and incorporates features such as apical assist for easier pipetting and basolateral access. To reduce the risk of monolayer contamination, the membrane plates have feet to elevate the plate above the work surface when disassembled from the feeder tray. Tear drop-shaped receiver wells eliminate air bubbles as plates are assembled.

Millicell plate are optimized to grow and sustain high integrity cell monolayers. Cells grown on Millicell plate membranes grow better than on plastic because the cells are nourished from both the apical and basolateral sides. Cell growth and function more closely mimic in vivo. Millicell plates are designed for analysis as well as cell growth and can be used manually or with automated cell seeding, feeding and washing systems

With this complete system, you can grow, feed, and analyze cells in one membrane-bottom plate. The plate can be used with either a single-well or a multi-well feeding tray. At the time of transport analysis, the plate is simply transferred to a multi-well transport tray for analysis.

This streamlined design enhances functionality with:

- Seed-and-feed systems

- Automated liquid handling systems and span pipettes

- Basolateral and apical access to cells

- Transepithelial electrical resistance (TEER) measurement systems

Packaging

Bulk packed

Components

96-well cell culture plate(5), 96-well receiver tray(10), lid(5)

Physical form

Color Code: Clear

Other Notes

Automation Compatible: Yes

Legal Information

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

Protocols

Toluidine blue stains the nuclear material in tissues with a high DNA and RNA content and is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.

This protocol covers 3 modes for the microscopic examination of cell samples.

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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