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PTHT24H48

Millipore

Millicell® 24 well hanging cell culture inserts

pore size 0.4 μm, translucent PET membrane, diam. 6.5 mm, Tissue Culture (TC)-treated surface, sterile

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Synonym(s):
cell culture inserts, membrane insert, plate inserts

material

polystyrene housing
translucent PET membrane

Quality Level

sterility

γ-irradiated
sterile

feature

100x106 pores/cm2

packaging

pack of 48 ea (individually blister packaged)

parameter

max. temp. at 37 °C

technique(s)

cell attachment: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

H

15.5 mm

diam.

6.5 mm

insert maximum volume

0.4 mL

insert working volume

0.2 mL

membrane thickness

9-16 μm

size

24 wells

surface area

0.3 cm2

color

translucent membrane

pore size

0.4 μm

binding type

Tissue Culture (TC)-treated surface

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This Item
PTEP24H48PTSP24H48PTMP24H48
sterility

sterile, γ-irradiated

sterility

γ-irradiated, sterile

sterility

γ-irradiated, sterile

sterility

γ-irradiated, sterile

pore size

0.4 μm

pore size

8.0 μm pore size

pore size

3.0 μm pore size

pore size

5.0 μm

binding type

Tissue Culture (TC)-treated surface

binding type

Tissue Culture (TC)-treated surface

binding type

Tissue Culture (TC)-treated surface

binding type

Tissue Culture (TC)-treated surface

packaging

pack of 48 ea (individually blister packaged)

packaging

pack of 48 ea (individually blister packaged)

packaging

pack of 48 ea (individually blister packaged)

packaging

pack of 48 ea (individually blister packaged)

size

24 wells

size

24 wells

size

24 wells

size

24 wells

General description

Millicell® Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has an high pore density translucent 0.4 μm pore size membrane and fits in most 24-well plastic tissue culture plates.

The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.

Application

  • Cell Proliferation
  • Cell Surface Receptors
  • Co-Culture
  • Epithelial Cell Growth
  • Feeder Layers
  • Fluorescent Detection / Immunohistochemistry
  • In Vitro Toxicology
  • Microbial Attachment
  • Polarized Protein Secretions
  • Polarized Uptake
  • Transport/Permeability

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.

Protocols

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

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