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RBN1MAG-31K

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MILLIPLEX® Rat Bone Magnetic Bead Panel 1 - Bone Metabolism Multiplex Assay

This MILLIPLEX MAP Rat Bone Magnetic Bead Panel 1 is a Bone Metabolism multiplex assay. The analytes available for this multiplex kit are: ACTH, Insulin, Leptin, Osteoprotegerin, DKK-1, SOST, FGF-23, PTH.

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eCl@ss:
32161000

Quality Level

species reactivity

rat

manufacturer/tradename

Milliplex®

assay range

accuracy: 88-105%
sensitivity: 0.8-42.2 pg/mL
standard curve range: 0.4-1,500 pg/mL
(PTH)

standard curve range: 10-40,000 pg/mL
(ACTH and OPG)

standard curve range: 12-50,000 pg/mL
(DKK-1)

standard curve range: 22-90,000 pg/mL
(FGF-23)

standard curve range: 31-125,000 pg/mL
(Insulin)

standard curve range: 5-20,000 pg/mL
(SOST)

standard curve range: 7-30,000 pg/mL
(Leptin)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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MBNMAG-41KRTHYMAG-30KRSHMAG-69K
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 88-105%, sensitivity: 0.8-42.2 pg/mL, standard curve range: 0.4-1,500 pg/mL
(PTH), standard curve range: 10-40,000 pg/mL
(ACTH and OPG), standard curve range: 12-50,000 pg/mL
(DKK-1), standard curve range: 22-90,000 pg/mL
(FGF-23), standard curve range: 31-125,000 pg/mL
(Insulin), standard curve range: 5-20,000 pg/mL
(SOST), standard curve range: 7-30,000 pg/mL
(Leptin)

assay range

accuracy: 84-94%, standard curve range: 10-40,000 pg/mL
(Leptin), standard curve range: 15-60,000 pg/mL
(DKK-1), standard curve range: 16-65,000 pg/mL
(OPG), standard curve range: 3-12,000 pg/mL
(SOST), standard curve range: 3-12,000 pg/mL
(TNFα), standard curve range: 37-150,000 pg/mL
(Insulin), standard curve range: 4-15,000 pg/mL
(ACTH), standard curve range: 7-30,000 pg/mL
(FGF-23), standard curve range: 7-30,000 pg/mL
(IL-6)

assay range

-

assay range

accuracy: 61%
(Corticosterone), accuracy: 83%
(ACTH), sensitivity: 0.9 pg/mL
(ACTH), sensitivity: 1.52 pg/mL
(Melatonin), sensitivity: 2914 pg/mL
(Corticosterone), standard curve range: 0.55-400 pg/mL
(Melatonin), standard curve range: 1.4-1,000 pg/mL
(ACTH), standard curve range: 686-500,000 pg/mL
(Corticosterone), inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Bone metabolism is the dynamic process of ongoing bone deposition and resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain this metabolic balance, these cells rely on complex signaling pathways involving hormones and cytokines to achieve the appropriate rates of growth and differentiation.

MILLIPLEX® Rat Bone Panel 1 is an 8-plex kit to be used for the simultaneous quantification of any or all of the following in rat serum and plasma samples: ACTH, OPG, Insulin, Leptin, PTH, DKK1, SOST, and FGF23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Bone

Specificity

Cross Reactivty
The antibody pairs in each panel do not show cross reactivity to the other analytes within the panel

Application

  • Analytes: ACTH, DKK1, FGF-23, Insulin, Leptin, Osteoprotegerin (OPG), PTH, Sclerostin (SOST)
  • Recommended Sample Type: Rat serum or plasma
  • Recommended Sample Dilution: 25 μL per well of neat serum or plasma
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C is recommended; alternately incubate for 2 hours at room temperature (20-25°C).
  • Research Category: Bone
  • Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3


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Cidália Pereira et al.
Nutrients, 11(10) (2019-10-03)
Bone mineral density (BMD) and microstructure depend on estrogens and diet. We assessed the impact of natural mineral-rich water ingestion on distal femur of fructose-fed estrogen-deficient female Sprague Dawley rats. Ovariectomized rats drank tap or mineral-rich waters, with or without
Sok Kuan Wong et al.
International journal of environmental research and public health, 16(18) (2019-09-12)
A positive association between metabolic syndrome (MetS) and osteoporosis has been demonstrated in previous animal studies. The mechanisms of MetS in orchestrating the bone remodelling process have traditionally focused on the interactions between mature osteoblasts and osteoclasts, while the role
Philip E Brandish et al.
European journal of pharmacology, 724, 102-111 (2014-01-01)
Glucocorticoids are used widely in the treatment of inflammatory diseases, but use is accompanied by a significant burden of adverse effects. It has been hypothesized that gene- and cell-specific regulation of the glucocorticoid receptor by small molecule ligands could be
Jennifer M Rojas et al.
Toxicologic pathology, 46(7), 777-798 (2018-10-23)
The obese rodent serves as an indispensable tool for proof-of-concept efficacy and mode-of-action pharmacology studies. Yet the utility of this disease model as an adjunct to the conventional healthy animal in the nonclinical safety evaluation of anti-obesity pharmacotherapies has not
João Paulo Steffens et al.
Journal of periodontology, 91(4), 545-553 (2019-08-08)
Sex hormone therapy has strict recommendations in the treatment of postmenopausal symptoms, in which testosterone (TES) replacement may play a potential role. However, it remains unclear whether TES affects the course of chronic inflammation and alveolar bone loss in females.

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