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RECYTMAG-65K

MILLIPLEX® Rat Cytokine/Chemokine Panel

Configurable Rat Cytokine/Chemokine 27-Plex Panel

Synonym(s):

rat cytokine multiplex panel, rat growth factor panel, rat inflammation biomarker panel, rat inflammatory cytokine panel

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

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species reactivity

rat

Quality Level

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

assay range

accuracy: 80-131%, standard curve range: 0.2-1,000 pg/mL
(EGF), standard curve range: 12.2-50,000 pg/mL
(GM-CSF, IL-1α, IL-2, IL-12 (p70), IL-18), standard curve range: 14.6-60,000 pg/mL
(Leptin, IFNγ, GRO/KC/CINC-1), standard curve range: 2.4-10,000 pg/mL
(MIP-1α, IL-1β, IP-10, Fractalkine, TNFα), standard curve range: 24.4-100,000 pg/mL
(LIX, MIP-2), standard curve range: 29.3-120,000 pg/mL
(MCP-1), standard curve range: 4.9-20,000 pg/mL
(G-CSF, Eotaxin, IL-4, IL-13, IL-5, VEGF, RANTES), standard curve range: 7.3-30,000 pg/mL
(IL-10, IL-17A), standard curve range: 73.2-300,000 pg/mL
(IL-6)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

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HCYP3MAG-63KHCYP2MAG-62KCCYTMG-90K-PX13
species reactivity

rat

species reactivity

human

species reactivity

human

species reactivity

canine

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 80-131%, standard curve range: 12.2-50,000 pg/mL
(GM-CSF, IL-1α, IL-2, IL-12 (p70), IL-18), standard curve range: 2.4-10,000 pg/mL
(MIP-1α, IL-1β, IP-10, Fractalkine, TNFα), standard curve range: 29.3-120,000 pg/mL
(MCP-1), standard curve range: 7.3-30,000 pg/mL
(IL-10, IL-17A), standard curve range: 0.2-1,000 pg/mL
(EGF), standard curve range: 24.4-100,000 pg/mL
(LIX, MIP-2), standard curve range: 73.2-300,000 pg/mL
(IL-6), standard curve range: 14.6-60,000 pg/mL
(Leptin, IFNγ, GRO/KC/CINC-1), standard curve range: 4.9-20,000 pg/mL
(G-CSF, Eotaxin, IL-4, IL-13, IL-5, VEGF, RANTES)

assay range

accuracy: 92-106%, standard curve range: 2.0-200,000 pg/mL

assay range

accuracy: 81-110%, sensitivity: 0.4-55.8 pg/mL, standard curve range: 1.0-100,000 pg/mL

assay range

accuracy: 91-109%, sensitivity: 13.6 pg/mL
(IFN-γ), sensitivity: 21.0 pg/mL
(MCP-1), sensitivity: 21.7 pg/mL
(IL-8), sensitivity: 3.2 pg/mL
(IP-10), sensitivity: 3.5 pg/mL
(IL-2), sensitivity: 3.7 pg/mL
(IL-6), sensitivity: 5.3 pg/mL
(KC-like), sensitivity: 5.8 pg/mL
(IL-18), sensitivity: 6.1 pg/mL
(TNFα), sensitivity: 7.5 pg/mL
(IL-7), sensitivity: 8.5 pg/mL
(IL-10), sensitivity: 9.0 pg/mL
(IL-15), sensitivity: 9.2 pg/mL
(GM-CSF), standard curve range: 12.2-50,000 pg/mL
(All analytes)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Rat cytokines and chemokines are critical signaling molecules that orchestrate immune responses, inflammation, and cellular communication in rodent models, making them essential biomarkers for preclinical research in drug development, toxicology studies, and disease pathogenesis. These small proteins, including interleukins, tumor necrosis factors, interferons, and chemotactic factors, regulate diverse physiological processes from wound healing and infection response to cancer progression and autoimmune disorders.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 27 analytes in rat serum, plasma, tissue/cell lysates, and cell culture supernatants.

Analytes included: EGF, Eotaxin/CCL11, Fractalkine, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, IP-10, Leptin, LIX, MCP-1, MIP-1α, MIP-2, RANTES, TNF-α, VEGF

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

  • Comprehensive Coverage: Includes 27 analytes, making it the largest rat cytokine/chemokine panel available for multiplexing.
  • Advanced Multiplexing: Uses Luminex® xMAP® technology to simultaneously quantify multiple biomarkers in a single sample.
  • High Accuracy: Assay accuracy ranges from 80–131%, ensuring reliable quantification.
  • Minimal Cross-Reactivity: Designed to avoid interference between analytes for cleaner data.
  • Flexible Sample Types: Verified for use with rat serum, plasma, and cell culture supernatants.
  • Configurable Kit Design: Choose analytes based on your research needs.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ginsenoside Rd maintains adult neural stem cell proliferation during lead-impaired neurogenesis.
Wang, B; Feng, G; Tang, C; Wang, L; Cheng, H; Zhang, Y; Ma, J; Shi, M; Zhao, G
Neurological Sciences null
A guinea pig model of Zika virus infection.
Kumar M, Krause KK, Azouz F, Nakano E, Nerurkar VR.
Virology Journal null
Mariano Guardia Clausi et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 37(3), 787-800 (2016-03-18)
Neuroinflammation subsequent to developmental brain injury contributes to a wave of secondary neurodegeneration and to reactive astrogliosis that can inhibit oligodendrocyte progenitor differentiation and subsequent myelination. Here we evaluated the therapeutic efficacy of a small molecule antagonist for a TGFß
?1-Antitrypsin inhibits ischemia reperfusion-induced lung injury by reducing inflammatory response and cell death.
Gao, W; Zhao, J; Kim, H; Xu, S; Chen, M; Bai, X; Toba, H; Cho, HR; Zhang, H; Keshavjeel, S; Liu, M
The Journal of Heart and Lung Transplantation : the Official Publication of the International Society For Heart Transplantation null
Jodie L Morris et al.
PloS one, 14(12), e0226574-e0226574 (2019-12-27)
Phage therapy offers a potential alternate strategy for the treatment of peri-prosthetic joint infection (PJI), particularly where limited effective antibiotics are available. We undertook preclinical trials to investigate the therapeutic efficacy of a phage cocktail, alone and in combination with

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Questions

  1. Can you provide information on the composition of the wash buffer for the Cytokine Milliplex kits?

    1 answer
    1. The buffer formulation is proprietary. However, what can be shared is that it is a PBS-based buffer with detergents and 0.05% Proclin.

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