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RMYOMAG-88K

Millipore

MILLIPLEX® Rat Myokine Magnetic Bead Panel

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple myokine and cytokine biomarkers in rat serum and plasma samples.

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eCl@ss:
32161000

Quality Level

species reactivity

rat

manufacturer/tradename

Milliplex®

assay range

accuracy: 82%
(FGF21)

accuracy: 83%
(IL-15)

accuracy: 85%
(Fractalkine (FKN))

accuracy: 85%
(Irisin)

accuracy: 88%
(IL-6)

accuracy: 91%
(FSTL1)

accuracy: 94%
(LIF)

inter-assay cv: <20%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

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This Item
HMYOMAG-56KRTHYMAG-30KRBN1MAG-31K
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

species reactivity

rat

species reactivity

human

species reactivity

rat

species reactivity

rat

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

General description

Skeletal muscle is actively involved in the synthesis and secretion of many proteins collectively termed “myokines.” Muscle contraction during physical activity is an important activator for the release of myokines, which act in an autocrine and/or paracrine manner to regulate skeletal muscle growth. Myokines also act as endocrine hormones which mediate inter-organ crosstalk. Myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue as well as for profound changes in immune and neuron systems. Various rat disease models are now serving as exceptional research tools for myokine study.

MILLIPLEX® Rat Myokine Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture samples: Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fibroblast Growth Factor 21 (FGF21), Follistatin-Like Protein 1 (FSTL-1), Fractalkine (FKN / CX3CL1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN / GDF8), Osteocrin/Musclin (OSTN), Osteonectin (SPARC). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: Brain-Derived Neurotrophic Factor (BDNF), Erythropoietin (EPO), Fibroblast Growth Factor 21 (FGF-21), Follistatin-like Protein 1 (FSTL1), Fractalkine (FKN/CX3CL1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Growth/Differentiation Factor 8 (GDF8/Myostatin), Osteocrin (OSTN/Musclin), Secreted Protein Acid and Rich in Cysteine (Osteonectin/SPARC)
  • Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants and lysates
  • Recommended Sample Dilution: 25μL per well of 1:2 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism, Endocrine
  • Research Subcategory: Obesity, Metabolic Disorders Inflammation, Muscle Physiology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Standard curve range: See protocol

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Wesam F Farrash et al.
Frontiers in endocrinology, 12, 645881-645881 (2021-06-29)
Assuming myokines underlie some of the health benefits of exercise, we hypothesised that 'high responder trainer' (HRT) rats would exhibit distinct myokine profiles to 'low responder trainers' (LRT), reflecting distinct health and adaptive traits. Blood was collected from LRT and
K Gawlinska et al.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 70(3) (2019-09-21)
A balanced maternal diet is necessary for the proper health and development of offspring. Recent clinical and preclinical studies have strongly indicated that maternal exposure to a high-fat diet (HFD) can have an irreversible impact on the structure and function

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