The specific plate utilized in the ImmunoSpot M200 kit is the S2EM004M99, MultiScreen-IP Filter Plate.
S2EM004M99
Multiscreen® 96 well Plate, hydrophobic PVDF membrane
pore size 0.45 μm, sterile
Synonym(s):
Hydrophobic 96-Well Plate, PVDF 96-Well Plate
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 ea | Estimated to ship TODAY | $454.00 |
About This Item
UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32039006
Material:
Durapore PVDF membrane, acrylic plate
Pore size:
0.45 μm pore size
Sterility:
sterile
$454.00
Estimated to ship TODAYDetails
Quality Level
material
Durapore PVDF membrane, acrylic plate
description
Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.
sterility
sterile
usage
Manual
packaging
pkg of 10 plates
manufacturer/tradename
MultiScreen®
technique(s)
ELISPOT: suitable, cell based assay: suitable
filtration area
0.28 cm2
plate size
96 wells
working volume
50-250 μL
matrix
Immobilon®-P
pore size
0.45 μm pore size
compatibility
manifold only
shipped in
ambient
storage temp.
10-30°C
General description
Filter Code: IP
Application
Physical form
Color Code: White
Other Notes
Automation Compatible: No
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| manufacturer/tradename MultiScreen® | manufacturer/tradename MultiScreen® | manufacturer/tradename MultiScreen® | manufacturer/tradename MultiScreen® |
| sterility sterile | sterility non-sterile | sterility non-sterile | sterility sterile |
| material Durapore PVDF membrane, acrylic plate | material Durapore PVDF membrane, clear acrylic plate, flat bottom wells | material Durapore PVDF membrane, flat bottom wells, white Barex® plate (co-polymer) | material Durapore PVDF membrane, flat bottom wells, white acrylic plate |
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| working volume 50-250 μL | working volume 50-250 μL | working volume 50-250 μL | working volume 50-250 μL |
| packaging pkg of 10 plates | packaging pkg of 50 plates | packaging pkg of 10 plates | packaging pkg of 10 plates |
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Protocols
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Articles
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
Related Content
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.
Sung-Ho Chang et al.
Cells, 9(5) (2020-05-24)
We investigated the immunogenicity of allogeneic human adipose-derived mesenchymal stem cells (ADSCs) through the production of alloreactive-CD8 T and -memory CD8 T cells, based on their human leukocyte antigen (HLA) expression. In surface antigen analysis, ADSCs do not express co-stimulatory
Sarah Côté-Bigras et al.
Clinical endocrinology, 80(2), 296-300 (2013-06-06)
While thyrotrophin receptor (TSHR) is recognized as the main autoantigen in Graves' disease (GD), the actual antigen specificity of T cells that infiltrate the thyroid and the orbit is unknown. Identifying T cell responses to TSHR peptides has been difficult
Daniela Frasca et al.
Vaccine, 34(25), 2834-2840 (2016-04-25)
The success of a vaccine in inducing a protective antibody response depends on the longevity of both long-lived plasma cells (PC) and memory B cells. We have previously shown that the in vivo antibody response to a new influenza vaccine
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S2EM004M99 | 04053252667800 |
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Which specific plate is utilized in the ImmunoSpot M200 kit?
1 answer-
Helpful?
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Can S2EM004M99 be utilized with Biosys Bioreader 7000 for Elispot reading?
1 answer-
The Biosys Bioreader 7000 has different versions, so it is important to confirm the specific model. If you have a 7000 V, it reads from above and will work with plates that need to be read from above. Regarding the specific plate S2EM004M99, it also needs to be read from above, making it compatible with the 7000 V model.
Helpful?
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