You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.
Select a Size
| Pack Size | SKU | Availability | Price |
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About This Item
Quality Level
material
PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing
product line
Stericup®
feature
hydrophilic
packaging
pack of 12 ea
parameter
max. temp. at 45 °C
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
164 mm
diam.
12.5 cm
filter capacity
250 mL
filter diam.
73 mm
filtration area
40 cm2
matrix
Durapore®
pore size
0.22 μm pore size
fitting
inlet funnel, outlet flask (Receiver)
shipped in
ambient
storage temp.
no temp limit
General description
Stericup® and Steritop® vacuum sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Stericup® 0.22 μm pore size polyvinylidene fluoride (PVDF) membrane filter unit combines a 250 mL bottle-top with a 250 mL receiver flask and cap for processing and storage.
Application
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
- The Quick Release filter funnel disconnects from the receiver bottle with just a quarter turn—reducing the likelihood of spillage and making it easier to manipulate on the bench or under the hood.
- Bold product identification on the Steritop® filter funnel plainly displays membrane characteristics, including composition and pore size, for rapid, application-appropriate product selection.
- Click seal cap is flanged and modified to enable a firm grip in wet or dry conditions—and a tactile stop confirms secure closure. Worry less about the possibility of spilling or contamination of sterilized contents.
- Frosted, ample writing surface on the receiver bottle and lighter cap color facilitate clear labelling to improve legibility and reduce unreadable smears.
- Click seal cap rests on the side to avoid risk of contamination.
- Receiver bottles are stackable to save storage space.
Legal Information
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This Item | |||
|---|---|---|---|
| filter capacity 250 mL | filter capacity 500 mL | filter capacity 500 mL | filter capacity 1000 mL |
| pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size |
| material PVDF membrane, polystyrene funnel(s), polystyrene bottle, polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing |
| packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea |
| fitting inlet funnel, outlet flask (Receiver) | fitting inlet funnel, outlet flask (Receiver) | fitting inlet funnel, outlet flask (Receiver) | fitting inlet funnel, outlet flask (Receiver) |
| feature hydrophilic | feature hydrophilic | feature hydrophilic | feature hydrophilic |
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Certificates of Analysis (COA)
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Articles
Select the right sterile filter by media, volume, and application.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S2GVU02RE | 04054839116933 |
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Can Stericup® bottles be placed inside freezers for long-term storage of media?
1 answer-
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What is the maximum vacuum pressure for filtration?
1 answer-
700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
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The User Guide says “Use only glass or plastic bottles designed for vacuum applications”. Can you specify which bottles are compatible?
1 answer-
Steritop® Quick Release bottle top units require a vacuum-rated receiver bottle with either a GL33 or GL45 mm closure thread as specified. Steritop® E bottle top units require a vacuum-rated receiver bottle with a GL45 mm closure thread. Bottles no larger than 2 L should be used.
Glass bottles, GL33:
• Wheaton media bottles (glass), 500 mL (Product Z364886)
• Wheaton media bottles (glass), 1 L (Product Z364894)
Glass bottles, GL45
• Corning® glass media storage bottle, 125 mL (Product CLS1399125)
• Corning® glass media storage bottle, 250 mL (Product CLS1399250)
• Corning® glass media storage bottle, 500 mL (Product CLS1399500)
• Corning® glass media storage bottle, 1 L (Product CLS13991L)
• Pyrex® round media storage bottle, 500 mL (Product CLS1395500)
• Pyrex® round media storage bottle, 1 L (Product CLS13951L)
• KIMBLE® KIMAX® Media bottle, 250 mL (Product DWK14395250)
• KIMBLE® KIMAX® Media bottle, 500 mL (Product DWK14395500)
• KIMBLE® KIMAX® Media bottle, 1 L (Product DWK143951000)
Plastic (pre-sterile) bottles, GL45:
• Stericup® Quick Release Receiver flask, 250 mL (Product S200B02RE)
• Stericup® Quick Release Receiver flask, 500 mL (Product S200B05RE)
• Stericup® Quick Release Receiver flask, 1000 mL (Product S200B10RE)Helpful?
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What is the chemical compatibility of the various Stericup® and Steritop® filters?
What is the chemical compatibility of the various Stericup® and Steritop® filters?
1 answer-
Please refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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What Bacterial Endotoxins Test is used?
1 answer-
The test method used complies with USP 85 and EP 2.6.14 to ensure the absence of endotoxin in our products.
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Can Stericup® and Steritop® filters be reused?
1 answer-
All Stericup® and Steritop® filters were developed for single use, disposable processing.
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Which type of pump should be used with Stericup® and Steritop® vacuum filters?
Which type of pump should be used with Stericup® and Steritop® vacuum filters?
1 answer-
We recommend our Chemical Duty Pump: https://www.sigmaaldrich.com/product/mm/wp6111560
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What tubing can be used with Stericup® and Steritop® vacuum filters?
What tubing can be used with Stericup® and Steritop® vacuum filters?
1 answer-
Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004
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Can we filter oil-type liquids with Stericup® filters?
Can we filter oil-type liquids with Stericup® filters?
1 answer-
Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?
1 answer-
No, all Stericup® and Steritop® devices are Research Use Only.
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