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S4030

Millipore

Dextran Sulfate 50% Solution

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Synonym(s):
dextran sulfate buffer, hybridization accelerant, hybridization additive, northern blot hybridization component, southern blot hybridization component
eCl@ss:
32160405

packaging

pkg of 100 mL

Quality Level

manufacturer/tradename

Chemicon®

technique(s)

hybridization: suitable (nucleic acid)

shipped in

ambient

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This Item
S4031S4117H0784
Millipore

Millipore

S4117

Formamide, Deionized

Hybri-slips L × W × thickness 60 mm × 24 mm × 0.25 mm

Sigma-Aldrich

H0784

Hybri-slips

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Grace Bio-Labs 716024

technique(s)

hybridization: suitable (nucleic acid)

technique(s)

hybridization: suitable (nucleic acid)

technique(s)

hybridization: suitable

technique(s)

-

shipped in

ambient

shipped in

ambient

shipped in

wet ice

shipped in

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

General description

Dextran Sulfate (MW >500,000) is used as an additive in probe hybridization solutions. Its presence accelerates the rate of hybridization by 10 fold. The addition of Dextran Sulfate favors the formation of probe networks, resulting in greater sensitivity during membrane-bound nucleic acid analysis. The product is supplied as a 50% (w/v) solution.

Application

Dextran Sulfate - 50% Solution

To prepare a solution containing 10% Dextran Sulfate, add 8 ml of hybridization ingredients to 2 ml of 50% Dextran Sulfate Solution.



See the "Hybrisol Nomograph for Dextran Sulfate" link located under the "Protocols" tab at catalog number S4030 product specific page on www.chemicon.com for determining the proper concentration of dextran sulfate needed for various hybridization condition guidelines.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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