S7107

Sigma-Aldrich

ApopTag TdT Enzyme

ApopTag Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection.

eCl@ss:
32161000

Quality Level

manufacturer/tradename

ApopTag
Chemicon®

shipped in

dry ice

General description

ApopTag Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag In Situ Apoptosis Detection Kits (Catalog #s: S7100, S7101, S7110, S7111, S7160 and S7165).
ApopTag is a registered trademark of Serologicals Company.

Packaging

300 μL in 1000 units/mL
1000 units/mL

Application

Materials Provided:
Each vial contains 300 μl of ApopTag Terminal Transferase (TdT) Enzyme.
Instructions for Use

Prepare labeling mixture as follows:
77 μl ApopTag Reaction Buffer (S7105 or kit component # 02)
33 μl ApopTag TdT Enzyme
Mix well and keep on ice. This reagent may be prepared in advance and stored on ice for no more than 6 hours. The Reaction Buffer - TdT Enzyme mixture is ready for use at the "APPLY WORKING STRENGTH TdT ENZYME" step in The Complete ApopTag Manual.
Warnings and Precautions :
ApopTag Terminal Transferase (TdT) Enzyme contains potassium cacodylate (dimethylarsinic acid) as a buffer. This material is harmful if swallowed; avoid contact with skin and eyes (wear gloves, glasses); wash areas of contact immediately.

Storage and Stability

Recommended Storage -15°C to -25°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazardEnvironment

Signal Word

Danger

Hazard Statements

WGK Germany

WGK 3

Certificate of Analysis

Certificate of Origin

Divya A Shiroor et al.
Current biology : CB, 30(11), 2166-2174 (2020-05-11)
Stem cells are continuously exposed to multiple stresses, including radiation and tissue injury. As central drivers of tissue repair and regeneration, it is necessary to understand how their behavior is influenced by these stressors. Planarians have an abundant population of...
Po-Nien Tsao et al.
Development (Cambridge, England), 136(13), 2297-2307 (2009-06-09)
Although there is accumulated evidence of a role for Notch in the developing lung, it is still unclear how disruption of Notch signaling affects lung progenitor cell fate and differentiation events in the airway epithelium. To address this issue, we...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.