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S7113

Sigma-Aldrich

DAPI/Antifade Solution, Ready to Use

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eCl@ss:
32161000
NACRES:
NA.47

manufacturer/tradename

Chemicon®

Quality Level

detection method

fluorometric

shipped in

dry ice

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This Item
MBD0020MBD0015D9542
DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

MBD0015

DAPI ready made solution

DAPI for nucleic acid staining

D9542

DAPI

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

detection method

fluorometric

detection method

-

detection method

-

detection method

-

General description

DAPI: 4′, 6-Diamino-2-phenylindole dihydrochloride. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 0.4μg/mL DAPI suspended in Antifade. The product is ready to add to fixed slides or cells cultures.

Application

A nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, metaphase chromosomes, or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent stains. Antifade prevents the permanent loss of fluorescence or fluorescent signal quenching due to photobleaching and prolonged exposure to high intensity light sources.

DAPI binds the minor groove of DNA at AT clusters and binds preferentially to ds DNA (Biochemistry 26:4545, 1987).

Materials Provided

Each vial contains 1 ml of DAPI/Antifade Solution

Warnings and Precautions

DAPI is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Use extreme caution. Wear gloves and a lab coat when handling. Follow your institution′s guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light.



Related Products

Antifade Solution S7114

Propidium Iodide Solution S7109

Propidium Iodide/Antifade Solution S7112

Physical form

Liquid PBS pH 8.0 containing ~50% glycerol and 0.4μg/mL of DAPI and 2-7mM phenylenediamine.

Storage and Stability

Store at -20ºC in undiluted aliquots for up to 1 year from date of receipt. Protect from light

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Creation of Biofunctionalized Micropatterns on Poly(methyl methacrylate) by Single-Step Phase Separation Method.
Ho QP, Wang SL, Wang MJ
ACS Applied Materials & Interfaces null
Observation of chondrocyte aggregate formation and internal structure on micropatterned fibroin-coated surface.
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Bio-medical materials and engineering null
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Mohammadhossein Khorraminejad-Shirazi et al.
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Mesenchymal stromal cell (MSC) stemness capacity diminishes over prolonged in vitro culture, which negatively affects their application in regenerative medicine. To slow down the senescence of MSCs, here, we have evaluated the in vitro effects of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), an
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Journal of cell science, 133(3) (2020-01-15)
Cell migration requires the coordination of multiple signaling pathways involved in membrane dynamics and cytoskeletal rearrangement. The Arf-like small GTPase Arl4A has been shown to modulate actin cytoskeleton remodeling. However, evidence of the function of Arl4A in cell migration is

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