S7855

CpGenome Rat Methylated Genomic DNA Standard

Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.

• eCl@ss: 32161000
• NACRES: NA.32

Properties

• Quality Level: 100
• species reactivity: rat
• manufacturer/tradename: CpGenome; Upstate^®
• application(s): genomic analysis
• shipped in: wet ice

Description

General description

The CpGenome Rat Methylated Genomic DNA Control is an ideal control or standard in DNA methylation studies. This methylated standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase followed by a treatment with a CpG Methylase. This process results in a DNA standard which contains ≥ 95% methylated cytosines across the genome in both heterochromatic and euchromatic regions.

Application

Research Category
Epigenetics & Nuclear Function

Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.

Quality

Quantitative Methylation Specific PCR (qMSP):
Bisulfite converted unmethylated and methylated rat genomic DNA are assessed for methylation status at various loci within the genome.

Physical form

5 µg DNA in buffer containing 10mM Tris-HCl pH 8.0, 0.1mM EDTA

Storage and Stability

Upon receipt aliqout and store at -20°C to avoid multiple freeze thaws which can fragment DNA. For best quality, limit freeze and thaw of the same –20°C DNA aliquot to no more than 3 times. For longer term storage, the DNA can be stored at -80°C. With proper storage and protection from excess freeze-thaw cycles, this product is stable for up to two years from the date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable