- S8001U
All Photos(1)
S8001U
CpGenome Human Non-Methylated DNA Standard Set
It is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.
All Photos(1)
Synonym(s):
Human Non-Methylated DNA Standard
eCl@ss:
32161000
Recommended Products
Quality Level
species reactivity
human
manufacturer/tradename
CpGenome
Upstate®
application(s)
genomic analysis
shipped in
dry ice
Related Categories
Compare Similar Items
View Full Comparison1 of 4
This Item | S8000-M | S8003 | 14-1067 |
|---|---|---|---|
| manufacturer/tradename CpGenome, Upstate® | manufacturer/tradename CpGenome, Upstate® | manufacturer/tradename CpGenome, Upstate® | manufacturer/tradename - |
| shipped in dry ice | shipped in - | shipped in dry ice | shipped in dry ice |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| species reactivity human | species reactivity mouse | species reactivity human | species reactivity - |
| application(s) genomic analysis | application(s) - | application(s) - | application(s) - |
General description
The CpGenome Human Non-Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. It is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ amplification kits.
Materials Provided:
One vial containing 5 µg (20 µL) of CpGenome Human Non-Methylated DNA Standard at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the CpGenome Human Non-Methylated DNA after bisulfite modification with the CpGenome Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the U primers generated product for this Standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Materials Provided:
One vial containing 5 µg (20 µL) of CpGenome Human Non-Methylated DNA Standard at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the CpGenome Human Non-Methylated DNA after bisulfite modification with the CpGenome Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the U primers generated product for this Standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
Please enter the Product Number and Lot Number.
Certificate of Quality
Please enter the Product Number and Lot Number.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service