SCC008

Sigma-Aldrich

ReNcell VM Human Neural Progenitor Cell LIne

ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

eCl@ss:
32011203
Pricing and availability is not currently available.

Quality Level

biological source

human

mfr. no.

Chemicon®
ReNcell

application(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

liquid nitrogen

General description

Product Source: Human ventral mesencephalon brain tissue
ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell VM retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell VM was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. In experiments performed by the ReNeuron Group plc, ReNcell VM can be differentiated in vitro to a high level of human dopaminergic neurons. Neurons differentiated from ReNcell VM have furthermore been shown to be electophysiologically active. ReNcell VM may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell VM cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures.

ReNcell Now Used in a 3D Cell Model of Alzheimer′s Disease!
Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease. Nature. 2014 Nov 13;515(7526):274-8.
(
Published in Nature)

Cell Line Description

Neural Stem Cells

Application

Research Category
Stem Cell Research

Components

> 1x106 viable ReNcell VM cells derived from human ventral mesencephalon brain tissue, cryopreserved. Store in liquid nitrogen.

Quality

Each lot of ReNcell VM cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities. Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.

Storage and Stability

When stored at the recommended storage conditions (liquid nitrogen), ReNcell VM cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Ahmed Elsherbini et al.
Acta neuropathologica communications, 8(1), 60-60 (2020-04-30)
Amyloid-β (Aβ) associates with extracellular vesicles termed exosomes. It is not clear whether and how exosomes modulate Aβ neurotoxicity in Alzheimer's disease (AD). We show here that brain tissue and serum from the transgenic mouse model of familial AD (5xFAD)...
A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line.
Song, Y; Subramanian, K; Berberich, MJ; Rodriguez, S; Latorre, IJ; Luria, CM; Everley et al.
Scientific data null
Teeo Tediose et al.
Nucleic acids research, 38(9), 2799-2812 (2010-01-27)
Non-malignant cells can be transformed via the activation of kinases that control degradation of neural-restrictive silencer factor (REST). Here, we identify a mechanism that contributes to the activation of genes, expression of which is controlled by responsive elements containing overlapping...
Transcriptional signature and memory retention of human-induced pluripotent stem cells.
Marchetto, Maria C N, et al.
Testing, 4, e7076-e7076 (2009)
Differential development of neuronal physiological responsiveness in two human neural stem cell lines.
Donato, R; Miljan, EA; Hines, SJ; Aouabdi, S; Pollock, K; Patel, S; Edwards, FA; Sinden, JD
BMC Neuroscience null
Articles
ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.
Read More
Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.
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Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
Read More
Products to enable the creation of neural models specific for your research.
Read More
Protocols
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
Read More
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
Read More

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