SCC041

C20A4 Human Chondrocyte Cell Line

Human

• Synonym(s): C-20/A4 ; ; C20/A4
• UNSPSC Code: 41106514
• NACRES: NA.81
• eCl@ss: 32011203
• Biological source: human

Properties

• Product Name: C20A4 Human Chondrocyte Cell Line, C20A4 Human Chondrocyte Cell Line is widely used as a model cell line for studying normal and pathological cartilage repair mechanisms related to chondrocyte biology and physiology.
• biological source: human
• Quality Segment: 100
• technique(s): cell culture | mammalian: suitable
• shipped in: ambient
• storage temp.: -140 to -196°C

Description

General description

The chondrocyte is the only specialized cell type present in articular cartilage. They produce secretions to support and repair the cartilage matrix. Human chondrocytes in mature articular cartilage are post-mitotic and terminally differentiated cells. Studies on human chondrocytes have been hampered by the difficulty in obtaining sufficient numbers of primary human chondrocytes from a single joint and by variabilities among donors due to factors such as age and medical conditions.

C-20/A4 cell line was established by transfecting primary cultures of rib chondrocytes from a 5-year-old male with vectors expressing origin-defective simian virus SV40 large T antigen.

Application

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Biochem/physiol Actions

Chondrocyte Cells

Preparation Note

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Analysis Note

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable