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SCC048

Sigma-Aldrich

N27 Rat Dopaminergic Neural Cell Line

The N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies.

Synonym(s):
Dopamine Neural Cells, 1RB3AN27 Cell Line
eCl@ss:
32011203
Pricing and availability is not currently available.

Quality Level

biological source

rat

application(s)

cell analysis: suitable (cell culture)
cell culture | stem cell: suitable

shipped in

liquid nitrogen

Related Categories

General description

Parkinson’s disease is a neurodegenerative disorder of the central nervous system that affects more than 6 million people worldwide. The motor symptoms of Parkinson′s disease result from the death of dopamine generating cells in the substantia nigra, a region of the midbrain. Numerous efforts have been made to slow the loss or replace these dopamine producing neurons in vivo.
The N27 rat dopaminergic neuron cell line was harvested from E12 rat mesencephalic tissue and was transfected with SV40 to immortalize the cell line. The N27 cell line, when injected into the striata of 6-hydroxydopamine-lesioned rats (an animal model of PD) caused a time-dependent improvement in neurological deficits. This immortalized cell line has been carefully characterized in studies of dopamine biosynthesis, neurotoxicity and used as a dopaminergic neuron model for in vitro and in vivo studies.

Cell Line Description

Neural Stem Cells

Neural Lineage Cells

Application

Research Sub Category
Neural Stem Cells

Neurochemistry & Neurotrophins

Neurodegenerative Diseases

Neuroregenerative Medicine
Research Category
Stem Cell Research

Neuroscience
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are negative for mycoplasma contamination.

Storage and Stability

N27 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.
Certificate of Analysis
Certificate of Origin
Establishment and characterization of immortalized clonal cell lines from fetal rat mesencephalic tissue.
Prasad, K N, et al.
In Vitro Cellular & Developmental Biology. Animal, 30A, 596-603 (1994)
M Terashvili et al.
Neuroscience, 223, 68-76 (2012-08-07)
Astrocytes perform several functions that are essential for normal neuronal activity. They play a critical role in neuronal survival during ischemia and other degenerative injuries and also modulate neuronal recovery by influencing neurite outgrowth. In this study, we investigated the...
Characterization and transplantation of two neuronal cell lines with dopaminergic properties.
Adams, F S, et al.
Neurochemical Research, 21, 619-627 (1996)
E D Clarkson et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(3), 1265-1270 (1998-03-14)
The replacement of dopamine (DA) by DA neuron transplants in the treatment of advanced Parkinson disease (PD) is a rational approach. Because of limitations associated with fetal tissue transplants, a clone (1RB3AN27) of simian virus 40 large tumor antigen (LTa)...
PKC? mediates paraquat-induced Nox1 expression in dopaminergic neurons.
Cristov?o, Ana Clara, et al.
Biochemical and biophysical research communications, 437, 380-385 (2013)
Articles
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