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SCC064

Sigma-Aldrich

LX-2 Human Hepatic Stellate Cell Line

The LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them a highly suitable model of human hepatic fibrosis.

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Synonym(s):
HSC, Perisinusoidal cells, Ito cells, Hepatic lipocytes, Hepatic pericytes
eCl@ss:
32011203
NACRES:
NA.75

biological source

human

Quality Level

technique(s)

cell culture | stem cell: suitable

shipped in

liquid nitrogen

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SCC043SCC109SCC163
technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell based assay: suitable, cell culture | mammalian: suitable

shipped in

liquid nitrogen

shipped in

ambient

shipped in

dry ice

shipped in

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

General description

Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.

Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.

Application

Research Category
Infectious Diseases

Inflammation & Immunology

Stem Cell Research

Components

1) ≥1X106 viable LX-2 Human Hepatic Stellate Cells: (Catalog No. SCC064). Store in liquid nitrogen.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negatrive for mycoplasma contamination.

Storage and Stability

LX-2 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality. LX-2 cells have been successfully expanded past passage 50 in culture.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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Isolated hepatic lipocytes and Kupffer cells from normal human liver: morphological and functional characteristics in primary culture.
Friedman, S L, et al.
Hepatology, 15, 234-243 (1992)
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Rationale: Fibrosis is a pathologic condition of abnormal accumulation of collagen fibrils. Collagen is a major extracellular matrix (ECM) protein synthesized and secreted by myofibroblasts, composing mainly (Gly-X-Y)n triplet repeats with >30% Gly residue. During fibrosis progression, myofibroblasts must upregulate
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Glucosylceramide (GluCer) was accumulated in sphingomyelin synthase 1 (SMS1) but not SMS2 deficient mouse tissues. In current study, we studied GluCer accumulation-mediated metabolic consequences. Livers from liver-specific Sms1/global Sms2 double-knockout (dKO) exhibited severe steatosis under a high-fat diet. Moreover, chow
Ching-Fen Wu et al.
Journal of cellular biochemistry, 114(3), 541-550 (2012-09-11)
Hepatitis C virus core protein (HCVcp), which is secreted by infected cells, is reported as an immunomodulator in immune cells. However, the effects of HCVcp on hepatic stellate cells (HSCs), the key cells in liver fibrosis, still remain unclear. In
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