Microglia are the primary immune cells of the central nervous system (CNS), and accounts for 10-15% of all cells found within the brain. Microglial play an important role in maintaining the health of the CNS by the removal of pathogens, infectious agents and damaged cells through phagocytosis. Microglial have also been extensively studied for their harmful roles in a variety of neurodegenerative disease including Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
The HAPI Rat Microglial Cell Line is a spontaneously immortalized cell line derived from primary microglia-enriched cultures prepared from 3-day-old rat brains (1). HAPI (highly aggressively proliferating immortalized) cells retain characteristics of microglia including marker expression, proinflammatory cytokine secretion in response to LPS, and phagocytic activity (1). These cells provide an alternative model to primary microglial cells.
1. Cheepsunthorn, P., Radov, L., Menzies, S., Reid, J., & Connor, J. R. (2001). Characterization of a novel brain‐derived microglial cell line isolated from neonatal rat brain. Glia, 35(1), 53-62.