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SCM013

Sigma-Aldrich

NDiff Neuro-27 Medium Supplement (100x)

NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants & other factors that has been developed for the in vitro propagation & maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium.

All Photos(1)
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

100

form

liquid

manufacturer/tradename

Chemicon®
Millipore

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)

shipped in

dry ice

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N6530SCM012SCM081
NDiff Neuro-27 Medium Supplement (100x) NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants & other factors that has been developed for the in vitro propagation & maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium.

Sigma-Aldrich

SCM013

NDiff Neuro-27 Medium Supplement (100x)

Sigma-Aldrich

Sigma-Aldrich

N6530

N1 Medium Supplement (100×)

NDiff Neuro-2 Medium Supplement (200x) NDiff Neuro-2 Medium Supplement is a serum-free, N2-like supplement for the in vitro differentiation of murine embryonic stem (ES) cells into post-mitotic neurons particularly via monolayer differentiation.

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SCM012

NDiff Neuro-2 Medium Supplement (200x)

N21 Medium Supplement (50X) The N21 Medium Supplement is an optimized serum-free supplement developed for isolation & expansion of neurons from mouse & rat hippocampus.

Sigma-Aldrich

SCM081

N21 Medium Supplement (50X)

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

dry ice

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

100

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Millipore Chemicon®

manufacturer/tradename

Millipore

input

sample type: mouse embryonic stem cell(s)

input

-

input

sample type neural stem cell(s)

input

sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)
sample type induced pluripotent stem cell(s)

General description

NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants and other factors that has been specifically developed for the in vitro propagation and maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium (Nichols J, 2006). This product can also be used to differentiate murine ES cells into post-mitotic neurons particularly via monolayer differentiation (Ying QL, 2003).

Physical form

NDiff Neuro-27 Medium Supplement is supplied as an aqueous solution. Optimal working concentration of the medium supplement must be determined for specific uses and cell types. In most instances, a 1:40-1:200 dilution is appropriate. Typically, Neuro-27 is diluted 1:100 for self renewal and neural differentiation of mouse ES cells.

Storage and Stability

Maintain at -20 °C until expiration date. Protect from light. Once thawed, aliquot unused portion and store at -20 °C until future use. Once added to the cell culture medium, the product is stable at 4 °C for four weeks.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Defined conditions for neural commitment and differentiation
Ying, Qi-Long and Smith, Austin G
Test, 365, 327-341 (2003)
Qi-Long Ying et al.
Nature biotechnology, 21(2), 183-186 (2003-01-14)
Mouse embryonic stem (ES) cells are competent for production of all fetal and adult cell types. However, the utility of ES cells as a developmental model or as a source of defined cell populations for pharmaceutical screening or transplantation is
Jennifer Nichols et al.
Methods in molecular biology (Clifton, N.J.), 329, 91-98 (2006-07-19)
The availability of murine embryonic stem (ES) cells has revolutionized the study of mammalian development and disease. We recently developed a culture medium that has enabled us to identify the essential signaling pathways required for maintenance of pluripotency in vitro.

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Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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