ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A Human Neural Progenitor Cells. Using ENStem-A Neuronal Differentiation Medium, relatively pure populations of neuronal cells (>90% bIII-tubulin positive) are obtained. Very low levels of astrocytes (<0.5% GFAP positive) are detected. Sufficient medium is supplied to provide for 100 separate reactions at 1 mL volume or 10 reactions at 10 mL volume.
The ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage.
Cell culture protocols for use with ENStem-A Human Neural Progenitor Cells are available in the datasheets that accompany the ENStem-A Human Neural Progenitor Expansion Kit (Part No. SCR055).
Each lot of media is tested for the optimal differentiation of ENStem-A Human Neural Progenitor Cells to a neuronal lineage.
Sterility Testing: Negative
Storage and Stability
ENStem-A Neuronal Differentiation Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the medium. Thawed medium should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.
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