SCM017

Sigma-Aldrich

ENStem-A Neuronal Differentiation Medium

The ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage.

eCl@ss:
32160801

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

application(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

dry ice

General description

ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A Human Neural Progenitor Cells. Using ENStem-A Neuronal Differentiation Medium, relatively pure populations of neuronal cells (>90% bIII-tubulin positive) are obtained. Very low levels of astrocytes (<0.5% GFAP positive) are detected. Sufficient medium is supplied to provide for 100 separate reactions at 1 mL volume or 10 reactions at 10 mL volume.

Application

The ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage.
Cell culture protocols for use with ENStem-A Human Neural Progenitor Cells are available in the datasheets that accompany the ENStem-A Human Neural Progenitor Expansion Kit (Part No. SCR055).

Quality

Each lot of media is tested for the optimal differentiation of ENStem-A Human Neural Progenitor Cells to a neuronal lineage.
Sterility Testing: Negative

Storage and Stability

ENStem-A Neuronal Differentiation Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the medium. Thawed medium should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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