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SCM075

Sigma-Aldrich

ROCK Inhibitor (Y-27632)

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Synonym(s):
Y-27632
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

form

liquid

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

General description

ROCK Inhibitor (Y-27632) is a selective, ATP-competitive inhibitor of Rho-associated protein kinase (ROCK) including p160Rock (Ki=140nM) and Rock-II. Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contraction. One-hour treatment with 10uM Y-27632 blocks apoptosis of dissociated cultured human embryonic stem cells (hESCs), increases survival and cloning efficiency of hESCs without affecting their pluripotency1. Recently, some reports showed that the application of Y-27632 and Accutase (Cat. No. SCR005), a formulated mixture of digestive enzymes, enhances the post-thaw survival rate of single hESCs using conventional slow freezing and rapid thawing methods

Storage and Stability

Before Reconstitution: Maintain at -20ºC, protected from light for 2 years. After Reconstitution: 1 year at -20ºC, protected from light

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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