Small molecules targeting specific signaling pathways have been shown to enhance the efficiency and/or replace transcription factors involved in somatic cell reprogramming. EMD Millipore has screened multiple chemical compounds and selected the compounds in this kit based on their effects on enhancement of reprogramming efficiency as assessed by the number and overall quality of mouse iPS colonies generated.
The Mouse iPS Reprogramming Boost Supplement contains three proprietary small molecules in amounts sufficient to supplement over 250 mL mouse ES/iPS cell maintenance medium. When used in conjunction with the Mouse STEMCCA lentivirus reprogramming kits (SCR530, SCR531), the Mouse iPS Reprogramming Boost Supplement enhanced the efficiency of mouse iPS colony formation by at least 3-fold. Mouse iPS Reprogramming Boost Supplement can be used to enhance reprogramming efficiency in somatic cell lines that may be more refractory to reprogramming. Mouse iPS cells generated through use of the Mouse iPS Reprogramming Boost Supplement display characteristic ES cell-like morphology, stained positive for alkaline phosphatase, expressed the correct mouse ES cell marker phenotype (SSEA-1 and Sox-2) and can be rapidly expanded in normal mouse ES cell culture conditions.
Kit contains sufficient quantities to supplement over 250 mL of medium.
1. TGF-ß RI Kinase Inhibitor II Supplement (1000X): (Part No. CS204458) One (1) vial containing 300 µL of the inhibitor in high quality DMSO.
2. GSK3ß Inhibitor Supplement (1000X): (Part No. CS204418) One (1) vial containing 300 µL of the inhibitor in high quality DMSO.
3. Methylation Modulator-A Supplement (1000X): (Part No. CS204419) One (1) vial containing 300 µL of the inhibitor in water.
Storage and Stability
Components of the Mouse iPS Reprogramming Boost Supplement are stable for at least 6 months from date of receipt when stored at -20°C. Upon first thaw, centrifuge the vial and gently mix the solution. Aliquot into smaller working volumes and freeze at -20°C or -80°C. Upon addition of the components of the Mouse iPS Cell Boost Supplement to the media, filter the supplemented media with a 0.22 µM filtration unit. Supplemented media is good for up to two weeks when stored at 2-8°C.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.