Traditionally, human embryonic stem cell (hESC) or induced pluripotent stem cell (iPSC) culture require mouse embryonic fibroblasts (MEF) support. This requirement makes the protocol complicated and variable. MEF conditioned medium (MEFCM) contains all the growth factors needed in hESC/iPSC culture, eliminating the routine preparation of feeder cells. It is a ready-to-use product for the feeder-free culture of human ES/IPS cells.
Each batch of EmbryoMax MEF Conditioned Media has been tested for hES/iPS cells pluripotency. The media requires an additional 10 ng/ml of bFGF to be added once thawed before use.
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
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12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
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