Once isolated, organoids are cultured in specialized media embedded in a rich extracellular matrix (ECM) basement membrane extract hydrogel such as Growth Factor Reduced Matrigel. Media is replenished every other day and cells are passaged once per week (7-12 days) before cells become too large or necrotic. Cells can be passaged in either small clump fragments or as single cells using mechanical dissociation/enzyme-free passaging reagents. Depending on organoid type and confluency, split ratios of roughly 1:3-1:4 can be used when passaging organoids. For sensitive organoid types, ROCKi (Y27632) (SCM075) can be added during passaging to help in promoting cell viability.
SCM301
3dGRO® Organoid Freeze Medium
Optimized cryopreservation media for multiple organoids cell types.
Synonym(s):
Organoid cryopreservation medium
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| Size/SKU | Availability | Price |
|---|---|---|
50 mL | Check Cart for Availability | $253.00 |
About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
eCl@ss:
32160801
$253.00
Check Cart for Availability
form
liquid
technique(s)
cell culture | stem cell: suitable
General description
Organoids are in-vitro derived 3D cell aggregates derived from primary tissue or stem cells that are capable of self-renewal, self-organization and exhibit organ functionality. Organoids address the limitations of existing 2D model systems by providing:
• Similar composition and architecture to primary tissue: Organoids harbor small population of self-renewing stem cells (such as intestinal crypt stem cells) that can differentiate into cells of all major cell lineages, with similar frequency as in physiological conditions.
• Relevant models of in-vivo conditions: Organoids are more biologically relevant to any model system and are amenable to manipulate niche components and gene expression.
• Stable system for extended cultivation: Organoids can be cryopreserved as biobanks and expanded indefinitely by leveraging self-renewal and the differentiation capability of the stem cell populations.
Organoids can be cryopreserved using standard cell freezing techniques however freeze/thaw viability remains a challenge. The 3dGRO Organoid Freeze Medium is a proprietary optimized cryopreservation media for multiple organoids cell types (etc. mouse intestinal organoids, human colon and lung organoids). The media supports higher freeze/thaw viabilities compared to other commercial or homemade organoid freezing media formulations.
• Similar composition and architecture to primary tissue: Organoids harbor small population of self-renewing stem cells (such as intestinal crypt stem cells) that can differentiate into cells of all major cell lineages, with similar frequency as in physiological conditions.
• Relevant models of in-vivo conditions: Organoids are more biologically relevant to any model system and are amenable to manipulate niche components and gene expression.
• Stable system for extended cultivation: Organoids can be cryopreserved as biobanks and expanded indefinitely by leveraging self-renewal and the differentiation capability of the stem cell populations.
Organoids can be cryopreserved using standard cell freezing techniques however freeze/thaw viability remains a challenge. The 3dGRO Organoid Freeze Medium is a proprietary optimized cryopreservation media for multiple organoids cell types (etc. mouse intestinal organoids, human colon and lung organoids). The media supports higher freeze/thaw viabilities compared to other commercial or homemade organoid freezing media formulations.
Application
Optimized cryopreservation media for multiple organoids cell types.
Research Category
Cell Culture
Stem Cell Research
Cell Culture
Stem Cell Research
Preparation Note
Store the 3dGRO Organoid Freeze Medium at -20°C. Aliquot into smaller working aliquots before use to avoid reagent contamination issues.
Analysis Note
Appearance (color): Amber
Osmolality: 250-350 mOsm
pH: 6.8-7.1
Sterility Tested: No Growth/Pass
Bacterial & Fungal Tested: Passed
Osmolality: 250-350 mOsm
pH: 6.8-7.1
Sterility Tested: No Growth/Pass
Bacterial & Fungal Tested: Passed
Legal Information
3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description Optimized cryopreservation media for multiple organoids cell types. | description Chemically defined enzyme-free organoid dissociation solution. | description Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids. | description Adenocarcinoma, metastatic (liver), Colon, Age: 57, Male, Mutation: Not Available |
| form liquid | form liquid | form liquid | form - |
| technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture: suitable |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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How are organoids cultured?
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How do you stain organoids with antibodies?
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Organoids can be stained with antibodies for immunocytochemical (ICC)/Immunohistochemical (IHC) analysis using either whole-mount or paraffin embedding/sectioning techniques. It is critical to use antibodies prequalified for organoid analysis.
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How Do you Extract RNA/DNA from Organoids in Cultured in Matrigel?
1 answer-
Traditional DNA/RNA isolation kits can be used to prepare genomic material for sequencing (RNA-seq, scRNA-seq, WGS, CHIP-seq etc). For some applications with large organoids, tissue specific DNA/RNA extraction kits are recommended. TRI Reagent (T9424) can be added to the dissociated organoids before RNA extraction and can be stored at -80 °C until future use.
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