MilliporeSigma
All Photos(1)

Documents

SCM310

Sigma-Aldrich

3dGRO® Skin Differentiation Medium

Sign Into View Organizational & Contract Pricing

form

liquid

packaging

pkg of 500 mL

technique(s)

cell differentiation: suitable

impurities

<2 EU/mL endotoxin

pH

7.0-7.6

osmolality

326-360 mOsm

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SCM301C-23061SCM018
vibrant-m

SCM301

3dGRO® Organoid Freeze Medium

form

liquid

form

liquid

form

liquid

form

liquid

packaging

pkg of 500 mL

packaging

-

packaging

pkg of 500 ml

packaging

-

impurities

<2 EU/mL endotoxin

impurities

-

impurities

-

impurities

-

pH

7.0-7.6

pH

-

pH

-

pH

-

osmolality

326-360 mOsm

osmolality

-

osmolality

-

osmolality

-

General description

Human skin contains both dermal and epidermal layers of cells connected by basement membrane ECM proteins. Dermal layers contain nerves, hair follicles, blood vessels, and fibroblasts, etc. Epidermal layers contain the squamous epithelium which consists of the basal, spinous, granular and cornified layers and serves a barrier to the outside enviromenment including pathogens and infection. In normal skin, keratinocytes replicate in the basal/parabasal layer and differentiate into the above layers. In the granular layer, keratinocytes undergo senescence and cornify generating the cornified layer. Organotypic 3D skin models can be generated by differentiating primary human keratinocytes (PHKs) using transwell culture inserts (Millicell). Briefly, PHKs are seeded onto a dermal equivalent consisting of rat tail collagen and human fibroblasts. The whole assembly is cultured until the PHK monolayer becomes confluent and then raised to the medium-air interface for 10 or more days. PHKs then stratify and differentiate into terminally differentiated squamous epithelium. Recetly, these organotypic 3D skin models have been used by the cosmetic industry as an alternative to animal testing.

The 3dGRO® Skin Differentiation Medium is an optimized formulation that supports robust human keratinocyte differentiation using organotypic 3D cultures during the medium-air incubation step. The media produces complex stratified squamous epithelium containing both proliferating (BrdU+) and cornified (Filaggrin+) cell polulations within 10 days. The media is complete and does not require further supplementation.

Application

Optimized media that supports robust human keratinocyte differentiation creating organotypic 3D skin models producing complex stratified squamous epithelium.

Research Category:
Cell Culture

Quality

Appearance: Clear liquid/no particulates
pH: 7.0−7.6
Osmolarity: 326−360 mOsm
Sterility: No growth
Mycoplasma: Negative
Endotoxin: <2EU/mL
Functional: Pass

Storage and Stability

The 3dGRO® Skin Differentiation Medium should be stored in −20°C. After thawing, store at 4°C and use in 2 weeks.

Legal Information

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Protocols

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service