All Photos(1)

SCR138

Sigma-Aldrich

Animal Free Collagenase, Type C

Animal Free Collagenase, Type C, is designed to have collagenase and secondary proteases similar to Types III and IV collagenase.

Synonym(s):
MMP, Matrix metalloprotease
eCl@ss:
32160405

Quality Level

caseinase activity

≥150 caseinase units/mg

clostripain activity

≤3.0 clostripain units/mg

collagenase activity

≥10,000 U/vial

trypsin activity

≤0.1 trypsin units/mg

application(s)

cell analysis: suitable (cell harvesting)
cell culture | mammalian: suitable

shipped in

wet ice

Related Categories

General description

Product Source: Isolated and purified from Clostridium histolyticum cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2008 certified enviroment.
Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types I, II, III, and IV or chromatographically purified collagenase. EMD Millipore’s Animal Free Collagenase, Type C, is prepared and purified from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens, such as BSE,TSE and other mammalian viruses, must be prevented.

Application

Research Category
Stem Cell Research
Animal Free Collagenase, Type C is designed to have collagenase and secondary proteases similar to Types III and IV collagenase.
Animal Free Collagenase, Type C, is designed to have collagenase and secondary proteases similar to Types III and IV collagenase.
Research Sub Category
Enzymes & Biochemistry

Physical form

Product is diafiltered with water, filtered through a 0.22 micron membrane and lyophilized in vials.

Storage and Stability

Store at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service