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SCT033
BioTracker 520 Green Hypoxia Dye
Live cell imaging dye for hypoxia that increases in fluorescence intensity with decreasing oxygen levels.
All Photos(4)
Synonym(s):
Live cell hypoxia probe, Live cell imaging probe
eCl@ss:
32161000
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
Related Categories
General description
Hypoxia is defined as the reduction or lack of oxygen in organs, tissues, or cells. Hypoxia in living tissue closely relates to a wide array of pathologies, including cancer and ischemia. Existing hypoxia detection probes, such as pimonidazole, require cell fixation followed by immunostaining.
The BioTracker 520 Green Hypoxia Dye is a fluorescent imaging probe for the detection of hypoxia in living cells. This probe is as sensitive as pimonidazole and can be used for live cell fluorescence imaging and flow cytometry applications.
Spectral Properties
Absorbance: 498nm
Emission: 520nm
The BioTracker 520 Green Hypoxia Dye is a fluorescent imaging probe for the detection of hypoxia in living cells. This probe is as sensitive as pimonidazole and can be used for live cell fluorescence imaging and flow cytometry applications.
Spectral Properties
Absorbance: 498nm
Emission: 520nm
Application
Research Sub Category
Live Cell Dye
Live Cell Dye
Research Category
Cell Imaging
Cell Imaging
Live cell fluorescent imaging
Physical form
Lyophilized
Storage and Stability
Store BioTracker 520 Green Hypoxia Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
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Certificate of Quality
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Data Sheets
Quotes and Ordering
Articles
Hypoxia Detection Assays
Hypoxia detection assays to measure oxygen levels in both live and fixed cells and tissues.
Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform
Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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