MilliporeSigma
All Photos(4)

SCT033

Sigma-Aldrich

BioTracker 520 Green Hypoxia Dye

Live cell imaging dye for hypoxia that increases in fluorescence intensity with decreasing oxygen levels.

Synonym(s):
Live cell hypoxia probe, Live cell imaging probe
eCl@ss:
32161000

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

Related Categories

General description

Hypoxia is defined as the reduction or lack of oxygen in organs, tissues, or cells. Hypoxia in living tissue closely relates to a wide array of pathologies, including cancer and ischemia. Existing hypoxia detection probes, such as pimonidazole, require cell fixation followed by immunostaining.

The BioTracker 520 Green Hypoxia Dye is a fluorescent imaging probe for the detection of hypoxia in living cells. This probe is as sensitive as pimonidazole and can be used for live cell fluorescence imaging and flow cytometry applications.

Spectral Properties
Absorbance: 498nm
Emission: 520nm

Application

Research Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging

Physical form

Lyophilized

Storage and Stability

Store BioTracker 520 Green Hypoxia Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Articles

Hypoxia Detection Assays

Hypoxia detection assays to measure oxygen levels in both live and fixed cells and tissues.

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service