Optimization of dye concentration and incubation time may be necessary. An example, 5 μM of this product was used on HeLa cells at 37°C with 5% CO2 for 3 hours. See image below.
SCT041
BioTracker Green Copper Live Cell Dye
Live Cell imaging probe for detection of Copper (Cu+) ion in live cells.
Synonym(s):
Fluorescent Cu+ detection
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5 X 50 NMOL
$710.00
$710.00
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About This Item
NACRES:
NA.47
UNSPSC Code:
12352207
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Product Name
BioTracker Green Copper Live Cell Dye, Live Cell imaging probe for detection of Copper (Cu+) ion in live cells.
form
lyophilized
application(s)
cell analysis
detection method
fluorometric
Related Categories
1 of 4
This Item | SCT211 | SCT032 | SCT026 |
|---|---|---|---|
| form lyophilized | form lyophilized | form - | form - |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) - |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
General description
Copper is one of the trace metals required by living organisms and is an essential cofactor for enzyme activities of cytochrome c oxidase, superoxide dismutase, and tyrosinase. On the other hand, abnormal homeostasis of copper leads to severe disorders such as Menkes syndrome and Wilson′s disease, or may be related to Alzheimer′s disease and cancers.BioTracker Green Copper Live Cell Dye is a fluorescent probe to detect Cu+, which is a dominant redox state of copper in an intracellular reducing environment. The dye is a colorless material with almost no fluorescence. Upon the reaction with Cu+, its fluorescence intensity at 510 nm increases more than 100 times to that before the reaction. However, fluorescence increase is not observed in the presence of reactive oxygen species and other trace metals in cells including Cu2+, Mn2+, Co2+, Ni2+, Fe+, Fe2+ and Zn2+.Spectral Properties:Absorbance (max): 480nmEmission (max): 510nmResearch Category:Live Cell Dye
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
728.8 °F
flash_point_c
387.1 °C
Certificates of Analysis (COA)
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Hi, how long should we incubate the copper probe with the cells? Also, what are the recommended incubation conditions (e.g., temperature, duration, and any other specific requirements)?
1 answer -
Could you please provide information on the concentration and use of the Biotracker Green Copper Live Cell Dye (SCT041)?
1 answer-
The Biotracker Green Copper Live Cell Dye (SCT041) is provided as a lyophilized powder, hence no specific concentration is provided.
Preparation instructions are as follows:
1. Preparation of the reagent: CopperGREEN is a colorless solid. Before opening the vial, spin down the solid to the bottom by a microcentrifuge.
2. Warm the vial to room temperature and add 50 μl of DMSO to one vial to make a 1 mM stock solution. Dissolve the crystal completely by careful pipetting. The solution will also be a colorless liquid. Use a neutral buffer solution for dilution of the stock solution. It's important to note that CopperGREEN could be oxidized in acidic solutions.For further details, please refer to the article and internal information.
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What is the detection range of this probe for copper concentration?
1 answer-
Unfortunately, the minimum intracellular level of Cu+ that the probe can identify has not been established. The recommended usage concentration in culture medium is 5 uM. In the cell-free system, the probe can detect Cu+ at a concentration of more than 0.2 µM.
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