It is recommended to fix and permeabilize the cells before staining with the BioTracker membrane dye and prior to performing immunofluorescent staining. To prevent dye redistribution, avoid using detergents after the staining step.
SCT107
BioTracker 555 Orange Cytoplasmic Membrane Dye
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Synonym(s):
Lipophilic carbocyanine dye, Live cell imaging probe, Live cell membrane dye
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1 ML
$392.00
$392.00
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About This Item
NACRES:
NA.47
UNSPSC Code:
12352207
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Product Name
BioTracker 555 Orange Cytoplasmic Membrane Dye, Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
1 of 4
This Item | SCT108 | SCT106 | SCT113 |
|---|---|---|---|
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
Analysis Note
Spectral Properties
Absorbance: 549nm
Emission: 565nm
Absorbance: 549nm
Emission: 565nm
Application
Live cell fluorescent imaging
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Lipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 549nm
Emission: 565nm
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 549nm
Emission: 565nm
Physical form
Liquid
Preparation Note
Store BioTracker 555 Orange Cytoplasmic Membrane Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
55.4 °F - closed cup
flash_point_c
13 °C - closed cup
Certificates of Analysis (COA)
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Related Content
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If using this dye on paraformaldehyde-fixed cells alongside antibody-based immunofluorescent staining, when should membrane labeling be performed?
1 answer-
Helpful?
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In order to stain fixed cells, do we have to use PBS or other buffer at room temperature instead of culture medium at 37oC like Biotracker 400 Blue Cytoplasmic Membrane dye?
1 answer-
BioTracker 555 Orange Cytoplasmic Membrane Dye (Cat. no. SCT107) is a lipophilic carbocyanine dye. Lipophilic carbocyanine dyes like this product have also been used to stain cells or tissues after formaldehyde fixation.
For a detailed protocol of the assay, please see the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/876/849/sct107ds.pdfHelpful?
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