MilliporeSigma
All Photos(1)

Documents

SCT111

Sigma-Aldrich

BioTracker 405 Blue SE Cell Proliferation Kit

Live cell imaging cell proliferation kit used for fluorescent cell labeling in flow cytometry applications.

Sign Into View Organizational & Contract Pricing

Synonym(s):
Live cell imaging probe

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SCT110SCT135SCT109
detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

General description

BioTracker SE Cell Proliferation Kits diffuse passively into live cells and are used for long-term cell labeling. They are initially non-fluorescent esters of amine-reactive dyes, but are converted to fluorescent dyes by intracellular esterases. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell. Immediately after staining a single, bright fluorescent population will be detected by flow cytometry. Each cell division that occurs after labeling is revealed by the appearance of a successively dimmer fluorescent peak on a flow cytometry histogram (Fig. 1). Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. The staining can withstand fixation and permeabilization for subsequent immunostaining. Alternative applications of cell proliferation dyes include uniform labeling of cell cytoplasm for microscopy, or labeling cells for quantitation of cell number by microplate reader.

Spectral Properties
Absorbance: 408nm
Emission: 452nm

Application

Live cell fluorescent imaging
Live cell imaging cell proliferation kit used for fluorescent cell labeling in flow cytometry applications.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Components

1. 10 vials of BioTracker 405 Blue SE Dye (100nmol) (CS224590)

2. 1 vials of Anhydrous DMSO (500µL) (CS224578)

Quality

Spectral Properties
Absorbance: 408nm
Emission: 452nm

Physical form

Liquid

Storage and Stability

Store BioTracker 405 Blue SE Cell Proliferation Kit -20ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening. Ideally the 5 mM DMSO stock solution should be prepared on the day of use. Aliquots may be stored for later use, but activity may be reduced over time. The dyes should only be added to aqueous buffer immediately before staining.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service