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SE1M179M6

Millipore

Steriflip®-GV Sterile Centrifuge Tube Top Filter Unit

Sterile, 50 mL process volume, 0.22 µm pore size, PVDF membrane, radio-sterilized

eCl@ss:
32031602
NACRES:
NB.22

material

PVDF membrane
methacrylate-butadiene-styrene (MBS)
polypropylene

Quality Level

sterility

sterile

feature

holdup volume 0.6 mL
hydrophilic

manufacturer/tradename

Steriflip®

parameter

45 °C max. temp.
50 mL process volume

application(s)

sterile filtration: suitable

H

138 mm

diam.

6.4 cm

filter diam.

4 cm

filtration area

7 cm2

funnel capacity

50 mL

matrix

Durapore®

pore size

0.22 μm nominal pore size
0.22 μm pore size

test parameters

sample volume: 50 mL

fitting

double lead thread inlet (with vacuum port)
double lead thread outlet for 50 mL centrifuge tube

shipped in

ambient

Related Categories

General description

The Steriflip® filter unit is designed to work with standard 50 mL centrifuge tubes, which eliminates sample transfer. It is useful for sterilization of aqueous solutions (up to 50 ml), sterile filtration of small batches of culture media, sterile filtration of buffers with high-value protein or small molecule components, and cell isolation.

Application

Steriflip®-GV Sterile Centrifuge Tube Top Filter Unit has been used:
  • in the preparation of antibody-drug conjugates (ADCs)
  • to sterilize bovine serum albumin (BSA), papain, dispase, and DNase mix and glucose solution for tissue culture
  • to filter clarified supernatant in human immunodeficiency virus (HIV-1) fusion assay

Features and Benefits

  • Eliminates messy sample transfer via a simple vacuum-assisted, closed system
  • Can isolate cells from cellular debris using any of the various pore-sized nylon net filters
  • Ideal for sterilizing small batches of culture media
  • Attaches to any standard 50 mL centrifuge tube, eliminating unnecessary materials, time and risk of spillage

Legal Information

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Steriflip is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

Certificate of Origin

Antibody-Drug Conjugates (2013)
Marielle Cavrois et al.
Bio-protocol, 4(16) (2014-08-20)
The HIV-1 fusion assay measures all steps in the HIV-1 life cycle up to and including viral fusion. It relies on the incorporation of a β-lactamase Vpr (BlaM-Vpr) protein chimera into the virion and the subsequent transfer of this chimera...
Harish Babu et al.
Frontiers in neuroscience, 5, 89-89 (2011-08-04)
In vitro assays are valuable tools to study the characteristics of adult neural precursor cells under controlled conditions with a defined set of parameters. We here present a detailed protocol based on our previous original publication (Babu et al., 2007)...
Jerzy A Woznicki et al.
Cell death & disease, 11(1), 68-68 (2020-01-29)
Proteins of the BCL-2 family are evolutionarily conserved modulators of apoptosis that function as sensors of cellular integrity. Over the past three decades multiple BCL-2 family members have been identified, many of which are now fully incorporated into regulatory networks...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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