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ESGRO Complete Accutase

The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

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Synonym(s):
Stem Cell Tested Accutase
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

shipped in

dry ice

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ESGRO Complete Accutase

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manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type cardiac stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells

input

-

input

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

300

General description

ESGRO Complete Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium (Cat. No. SF001-500). Accutase does not contain mammalian or bacterial derived products.

Application

Cell Detachment:

1. Thaw Accutase® to room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add Accutase to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.

Physical form

Frozen sterile liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

1X Accutase® enzymes in Dulbecco′s PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.

Storage and Stability

Stable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase® may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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