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SF016

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ESGRO-2i Medium, 100ml

ESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β & Mek 1/2 inhibitors to enhance viability of mouse embryonic stem & iPS cells in the absence of serum & feeder cells. Supports a naive/ground pluripotent state.

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Synonym(s):
2i Naive ES/IPS Media
eCl@ss:
32160801

Quality Level

form

liquid

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

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SF001SCM001SCM130
ESGRO-2i Medium, 100ml ESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β & Mek 1/2 inhibitors to enhance viability of mouse embryonic stem & iPS cells in the absence of serum & feeder cells. Supports a naive/ground pluripotent state.

Sigma-Aldrich

SF016

ESGRO-2i Medium, 100ml

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

Quality Level

100

Quality Level

100, 300

Quality Level

300

Quality Level

100

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

input

-

General description

Two stages of mouse pluripotency have been stabilized to date through in vitro culture. ‘Naive’ represents the earliest stage, which can be enriched for a purer population, termed ‘ground state’. Naive most closely approximates embryonic epiblast in the preimplantation blastocyst. The primed state most closely approximates in vivo immediate postimplantation epiblast. There are clear differences between these stages in culture, expression, epigenetics (including appropriate X-inactivation) and developmental competence. The fact that naive mESCs can generate whole animals through tetraploid complementation while primed mESCs cannot, is a particularly compelling reason for heightened expectations from naive hESCs.
ESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β and Mek 1/2 inhibitors to enhance viability of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for cell expansion and growth at clonal density in serum-free conditions and supports a naive/ground pluripotent state.

Application

Suitable for ES and iPS cell derivation, passaging and maintenance. Media can be used on feeder dependent and independent pluirpotent stem cell lines.

Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete Accutase (Cat # SF006)
ESGRO Complete Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)

Components

1. ESGRO-2i Basal Medium with LIF: (Catalogue No. CS204463) One (1) 100 mL bottle containing basal medium supplemented with LIF, pH 7.1-7.3.

2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-050) One (1) 50 μL vial containing selective GSK3β inhibitor supplement.

3. Mek1/2 Inhibitor Supplement: (Catalogue No. CS204465) One (1) 10 μL vial containing selective Mek 1/2 inhibitor supplement.

Storage and Stability

• Upon receipt, store basal medium and inhibitor supplements in the dark at -20°C until use. Refer to lot expiration date on labels.
• After basal medium and inhibitor supplements are combined, store the complete ESGRO-2i medium in the dark at 4°C for up to 10 days. Do not refreeze.

Legal Information

cOmplete is a trademark of Roche

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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Articles

Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors.

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