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MilliporeSigma

SNAP2MB1

SNAP id® 2.0 Protein Detection System

Mini and MultiBlot

Synonym(s):

Protein detection system

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1 PKG

$2,400.00

$2,400.00


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About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

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Product Name

SNAP i.d. 2.0 Protein Detection System-Mini and MultiBlot (7.5 x 8.4 cm and 4.5 x 8.4 cm), Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane.

manufacturer/tradename

SNAP id®

technique(s)

western blot: suitable

compatibility

for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

storage temp.

room temp

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This Item
SNAP2MB3SNAP2MB2SNAP2MIDI
shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

manufacturer/tradename

SNAP id®

manufacturer/tradename

SNAP id®

manufacturer/tradename

SNAP id®

manufacturer/tradename

SNAP id®

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

compatibility

for use with Commercially available blocking reagents

compatibility

for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents

compatibility

for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents

compatibility

for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents

detection method

chemiluminescent

detection method

chemiluminescent, colorimetric, fluorometric

detection method

chemiluminescent, colorimetric, fluorometric

detection method

chemiluminescent, colorimetric, fluorometric

General description

This system includes the SNAP i.d. 2.0 Base, a Mini Blot Holding Frame,a MultiBlot Holding Frame, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start Guide

Application

Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane.
For use on immunoblots.

Other Notes

Replaces: WBAVDBASE

Legal Information

SNAP id is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zeina S Khan et al.
Biomicrofluidics, 13(3), 034110-034110 (2019-08-23)
Highly metastatic prostate cancer cells flowing through a microfluidic channel form plasma membrane blebs: they form 27% more than normal cells and have a lower stiffness (about 50%). Hypo-osmotic stress assays (with ∼ 50 % osmolarity) show 22% more blebbing
Morgane Agez et al.
Scientific reports, 9(1), 13675-13675 (2019-09-25)
CD20 is a B-lymphocyte specific integral membrane protein, an activated-glycosylated phosphoprotein expressed on the surface of B-cells and a clinically validated target of monoclonal antibodies such as rituximab, ocrelizumab, ofatumumab and obinutuzumab in the treatment of all B cell lymphomas
Amit Roshan et al.
Nature cell biology, 18(2), 145-156 (2015-12-08)
Single stem cells, including those in human epidermis, have a remarkable ability to reconstitute tissues in vitro, but the cellular mechanisms that enable this are ill-defined. Here we used live imaging to track the outcome of thousands of divisions in

Articles

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

Protocols

The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.

Related Content

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

Lo entendemos. Los Westerns pueden ser un dolor de muelas, y ciertamente hemos hecho nuestra parte. Así que, ¡comparte con nosotros tus feos borrones y recibe una sorpresa! Y aunque no podemos decirte de qué se trata -eso estropearía la sorpresa-, salvo que despertará la creatividad en el laboratorio.

There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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