ST1507

Sigma-Aldrich

Anti-AKR1B10 Mouse mAb (1A6)

liquid, clone 1A6, Calbiochem®

Synonym(s):
Anti-Aldo-Keto Reductase 1B10

storage conditions

-20C

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

1A6, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

General description

Recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.
Anti-AKR1B10. Mpuse monoclonal, clone 1A6 recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue. It is validated for ELISA, WB, ICC, and paraffin sections.
Purified mouse monoclonal antibody. Recognizes the ~37 kDa AKR1B10 protein.

Immunogen

NA
A recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein

Application




ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HepG2 cells, Human Colon tissue, HeLa cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Ahn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE56, 287.
Satow, R., et al. 2010. Clin. Cancer Res.16, 2518.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Jennifer A Harbottle et al.
Analytical biochemistry, 592, 113583-113583 (2020-01-17)
Improved health span and lifespan extension in a wide phylogenetic range of species is associated with the induction of the environmental cell stress response through a signalling pathway regulated by the transcription factor Nrf2. Phytochemicals which stimulate this response may...

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