Recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.
Anti-AKR1B10. Mpuse monoclonal, clone 1A6 recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue. It is validated for ELISA, WB, ICC, and paraffin sections.
Purified mouse monoclonal antibody. Recognizes the ~37 kDa AKR1B10 protein.
A recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)
Toxicity: Regulatory Review (Z)
In PBS, pH 7.2.
Following initial thaw, aliquot and freeze (-20°C).
HepG2 cells, Human Colon tissue, HeLa cells
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Ahn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE56, 287.
Satow, R., et al. 2010. Clin. Cancer Res.16, 2518.
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