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MilliporeSigma

ST1551

Sigma-Aldrich

Anti-GGT1 Mouse mAb (1F9)

liquid, clone 1F9, Calbiochem®

Synonym(s):

Anti-γ-Glutamyltransferase1

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100 μG
$622.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

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$622.00

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Technical Service
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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1F9, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1-5 µg/mL)
Paraffin Sections (3 µg/mL))

isotype

IgG2α

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GGT1(2678)

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Anti-GGT1 Mouse mAb (1F9) liquid, clone 1F9, Calbiochem®

ST1551

Anti-GGT1 Mouse mAb (1F9)

Anti-FGF8 Mouse mAb (2A10) liquid, clone 2A10, Calbiochem®

ST1630

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Anti-GGT6 antibody produced in rabbit

Anti-DYRK1A Mouse mAb (7D10) liquid, clone 7D10, Calbiochem®

ST1650

Anti-DYRK1A Mouse mAb (7D10)

species reactivity

human, mouse

species reactivity

mouse, human, rat

species reactivity

human

species reactivity

mouse, human, rat

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

Gene Information

human ... GGT1(2678)

Gene Information

mouse ... Fgf8(14179)

Gene Information

human ... GGT6(124975)

Gene Information

human ... DYRK1A(1859)
mouse ... Dyrk1A(13548)
rat ... Dyrk1A(25255)

clone

1F9, monoclonal

clone

2A10, monoclonal

clone

polyclonal

clone

7D10, monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

form

liquid

form

liquid

form

buffered aqueous glycerol solution

form

liquid

General description

Anti-GGT1, mouse monoclonal, clone 1F9, recognizes the ~60 kDa GGT1 protein in NIH3T3 cells and human testes tissue. It is validated for Western blotting & immunohistochemistry on paraffin sections.
Purified mouse monoclonal antibody. Recognizes the ~60 kDa GGT1 protein.
Recognizes the ~60 kDa GGT1 protein in NIH3T3 cells and human testes tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids 381-471 of human GGT1, expressed as a GST fusion protein

Application

Immunoblotting (1-5 µg/mL)Paraffin Sections (3 µg/mL)

Analysis Note

Positive Control
NIH3T3 cells, Human testes tissue

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. Can we use this antibody on bovine y-spermatozoa to bind surface protein of sperm cell ?

    1 answer

    1. The immunogen of ST1551 Sigma-Aldrich Anti-GGT1 Mouse mAb (1F9) is a recombinant polypeptide corresponding to amino acids 381-471 of human GGT1, expressed as a GST fusion protein. This sequence has 89% homology to bovine, so most likely the antibody will work in bovine, but we have not tested it in-house in bovine samples.

      It appears that ST1551 has been utilized with bovine spermatozoa in the papers referenced below. It is recommended to review these papers for detailed information regarding the experimental protocols and results.
      CD9-Positive Microvesicles Mediate the Transfer of Molecules to Bovine Spermatozoa during Epididymal Maturation
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681974/

      https://www.google.com/search?q=ST1551+bovine&rlz=1C1GCEA_enUS976US976&oq=ST1551+bovine&gs_lcrp=EgZjaHJvbWUyBggAEEUYOTIGCAEQRRg90gEIMzE5OWowajeoAgCwAgA&sourceid=chrome&ie=UTF-8#ip=1

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